白藜芦醇通过线粒体凋亡通路调节肺癌H460细胞增殖与凋亡的研究  

The research on Resveratrol regulates lung cancer H460 cells on proliferation and apoptosis by the mitochondrial signaling pathway

作　　者：谢兴[1] 钟卫权[1] 

机构地区：[1]惠州市第三人民医院心胸外科,广东惠州516000

出　　处：《现代肿瘤医学》2017年第21期3402-3406,共5页Journal of Modern Oncology

基　　金：惠州市科学计划项目(编号:20130802)

摘　　要：目的:探讨白藜芦醇(Resveratrol,Res)对肺癌H460细胞增殖与凋亡的影响并探讨其可能的机制。方法:分别用浓度为0、25、100、200μmol/L的Res作用于H460细胞48 h后,采用CCK-8法检测Res对H460细胞增殖的影响;流式细胞术检测细胞凋亡率;JC-1试剂盒检测细胞内线粒体膜电位水平;ATP试剂盒检测细胞内ATP水平;Western blot法检测细胞内Bcl-2、Bax和Cytochrome c蛋白表达情况。结果:与对照组比较,CCK-8及流式结果显示Res可抑制H460细胞的增殖,并使其凋亡率升高(P<0.01);JC-1及ATP检测结果显示Res可降低H460细胞内线粒体膜电位及ATP水平(P<0.01),且在该浓度范围内呈剂量相关性;Western blot检测发现随着Res浓度的增加,Bcl-2蛋白的表达水平下降,而Bax和Cytochrome c蛋白的表达水平升高。结论:实验浓度的Res可抑制H460细胞的增殖并诱导其凋亡,这一作用的机制可能与Res激活了线粒体凋亡通路进而诱发H460细胞凋亡有关。Objectlve：To observe the effect of Resveratrol on proliferation and apoptosis of lung cancer H460 cells and explore the possible mechanisms. Methods：H460 cells were treated with Res （0,25,100,200 μmol/L） for 48 hours. The cell proliferation was measured by eel1 counting Kit - 8, the apoptosis assays were performed by flow cytom- etry, the mitoehondrial membrane potential level was detected by JC - 1. The ATP levels in H460 cells were measured using an ATP assay kit, and the expression level of Bax, Bcl - 2 and Cytochrome c protein in H460 cells were analysed by Western blot. Results： Compared with the control group, treated with Res inhibiteyd the proliferation of H460 cells, and enhanced the apoptosis rate through the CCK - 8 and flow cytometry （ P 〈 0.01 ）, and the JC - 1 and ATP detec- tion showed that Res decreased the mitochondrial membrane potential and ATP levels in H460 cells in a dose - de- pendent manner（ P 〈 0.01 ）. Meanwhile, the expression level of Bcl - 2 was downregulated, however the Bax and Cyto- chrome were upregulated with the increase of the Res concentration. Conclusion： Res inhibited the proliferation and induced the apoptosis of H460 cells, and which was involved in mitoehondfial signaling pathway of apoptosis, poten- tially.

关 键 词：白藜芦醇 H460细胞 增殖 凋亡 

分 类 号：R734.2[医药卫生—肿瘤]