微小RNA-21、微小RNA-371-5p和微小RNA-378在3种消化道肿瘤的表达及其临床意义  

Expression patterns of microRNA -21, microRNA -371 -5p and microRNA -378 in three types of gastrointestinal tumors and their clinical significances

作　　者：陈香浏 徐志远[3,4] 程向东[4] 刘翔[2] 王久利[2] 洪莲莲 凌志强[1,2] 

机构地区：[1]温州医科大学第一临床医学院肿瘤外科,325035 [2]浙江省肿瘤医院浙江省肿瘤研究所,杭州310022 [3]浙江省肿瘤医院浙江省肿瘤外科,杭州310022 [4]浙江省中医药大学附属第一医院浙江省中医院肿瘤外科,杭州310006

出　　处：《中华实验外科杂志》2017年第10期1695-1698,共4页Chinese Journal of Experimental Surgery

基　　金：浙江省重大科技专项计划项目（2013C03044-4）;浙江省卫生高层次创新人才培养工程基金项目（浙卫发[2014]108号）;浙江省新世纪151人才工程重点层次项目（浙人社发[2014]150号）

摘　　要：目的探讨微小RNA（miRNA，miR）-21、miR-371-5p、miR-378在胃癌、肝癌和结直肠癌3种消化道肿瘤中的表达及临床意义。方法收集100例胃癌患者、50例肝癌患者、50例结直肠癌患者术前血清、术后及随访终点血清，Luminex液相芯片检测血清miRNA的表达，分析3种miRNAs在消化道肿瘤中的表达及临床意义。结果术前血清miR-21在胃癌、肝癌和结直肠癌复发组中表达中位数分别为5.55、6.39、5.46，显著高于未复发组（中位数分别为2.52、2.87、2.18），差异有统计学意义（均P＝0.000）。术前血清miR-371-5p在胃癌、肝癌复发组中表达中位数分别为7.29、2.11，显著高于未复发组（2.56、1.82），差异有统计学意义（均P＝0.000）；在结直肠癌中，复发组术前血清miR-371-5p表达中位数为0.33，显著低于未复发组（3.23），差异有统计学意义（P＝0.000）。术前血清miR-378在胃癌和结直肠癌复发组表达中位数分别为10.13、0.33，与未复发组中表达中位数（分别为3.23、0.45）比较差异有统计学意义（均P＝0.000）；肝癌中，术前血清miR-378在复发组和未复发组表达中位数分别为0.98、1.09，两者比较差异无统计学意义（P＝0.410）。3种miRNA的表达与3种消化道肿瘤M分期相关。miR-21表达随着3种肿瘤切除、复发而呈现动态变化，miR-371-5p、miR-378仅与胃癌呈现动态变化。miR-21、miR-371-5p的表达与3种肿瘤术后复发显著相关，miR-378的表达与胃癌和结直肠癌预后显著相关（均P＝0.000）。结论检测miR-21有助于评估消化道肿瘤非特异性预后，检测miR-371-5p和miR-378有助于评估胃癌和结直肠癌患者的预后。ObjectiveTo explore the expression patterns and clinical significance of microRNA （miRNA, miR）-21, miR-371-5p and miR-378 in gastric carcinoma, hepatocellular carcinoma and colorectal cancer. MethodsThe serum samples of 100 patients with gastric cancer, 50 patients with hepatocellular carcinoma, 50 patients with colorectal cancer were collected before and after operation, and at the follow-up endpoint. Luminex liquid microarray was used to detect the expression of serum miRNAs to analyze the expression patterns of three miRNAs in gastrointestinal tumors and their clinical significance. ResultsPreoperative serum miR-21 expression in gastric cancer recurrence group （median： 5.55）, liver cancer recurrence group （median： 6.39） and colorectal cancer recurrence group （median： 5.46） was significantly higher than that in those non-recurrence groups （median： 2.52, 2.87 and 2.18 respectively）, and the difference was statistically significant （all P=0.000）. Preoperative serum miR-371-5p expression in gastric cancer recurrence group （median： 7.29） and liver cancer recurrence group （median： 2.11） was significantly higher than that in those non-recurrence groups （median： 2.56 and 1.82, respectively）, and the difference was statistically significant （both P=0.000）. In colorectal cancer, the median of preoperative serum miR-371-5p expression in the recurrence group was 0.33, significantly lower than that in the non-recurrence group （median： 3.23）, and the difference was statistically significant （P=0.000）. The median of preoperative serum miR-378 expression in gastric cancer recurrence group and colorectal cancer recurrence group was 10.13 and 0.33 respectively, and that in those non-recurrence groups was 3.23 and 0.45 respectively, with statistically significant difference （both P=0.000）. The median of preoperative serum miR-378 expression in live cancer recurrence group and the non-recurrence group was 0.98 and 1.09 respectively, and there was no significant

关 键 词：消化道肿瘤 微小RNA 预后 

分 类 号：R735[医药卫生—肿瘤]