3个品种猪MEF2D基因启动子多态及其生物信息学分析  

The Polymorphisms and Bioinformatics of Promoter Region of MEF2D Gene in Congjiang Xiang Pig

作　　者：白优[1] 张勇[1] 陈祥[1,2] 张雄 李俊[1,3] 何琦[1] 杨红 陆静[1] 

机构地区：[1]贵州大学高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵州省动物遗传育种与繁殖重点实验室,贵州大学动物科学学院,贵州贵阳550025 [2]贵州省生猪健康养殖工程技术研究中心,贵州贵阳550025 [3]贵州省种畜禽种质测定中心,贵州贵阳550025

出　　处：《中国畜牧杂志》2017年第10期34-38,共5页Chinese Journal of Animal Science

基　　金：贵州大学引进人才科研项目[贵大人基合字2013(21)号];贵州省工程中心建设项目(黔科合农C字[2011]4022号)

摘　　要：研究以从江香猪、杜×长×大外三元杂交猪及贵州宗地花猪为研究对象,采用混合DNA池结合直接测序技术筛选猪MEF2D基因5'UTR及第1外显子区SNPs位点;同时利用生物信息学软件分析SNPs位点对核心启动子区、Cp G岛和转录因子结合位点的影响。结果表明:在猪MEF2D基因5'UTR区共筛查到4个SNPs位点,分别为A-511G、T-453A、T-242G和T-180A;生物信息学软件预测发现T-453A和T-242G位点附近有重要转录因子结合位点消失和新位点生成,据此推测猪MEF2D基因5'UTR区的T-453A和T-242G位点对调控启动子功能元件可能存在重要影响。The Congjiang Xiangpigs, the commercial crossbred pigs as well as the Guizhou Zongdi Huapigs as research animals, were used to screen the gene MEF2D of 5＇UTR and the SNPs sites in part first exon region by using DNA pool and PCR direct sequencing. Variety bioinformatics softwares were used to analyse the SNPs sites how to affect the core region of the promoter, transcription factors binding sites and CpG island. The results showed there were four SNPs sites in the 5＂UTR regions of MEF2D, including A-511G, T-453A, T-242G and T-180A. It was identified that various new transcription factors binding sites emerged and some significant transcription factors binding sites disappeared nearby the region at T-453A and T-242G site. So we speculated that T-453A and T-242G site locating MEF2D ＂ 5＂ UTR have a deep effect on regulating the functional element of promoter.

关 键 词：从江香猪 MEF2D基因 SNP 启动子 生物信息学 

分 类 号：S828.2[农业科学—畜牧学]