离子色谱-质谱联用法检测咖啡豆中的典型单糖和小分子寡糖  

Determination of representative monosaccharide and small molecule oligosaccharides in coffee beans by IC-MS

作　　者：张涛[1,2] 蔡峰[2,3] 崔鹤[2] 马继平 朱倩林[4] 胡东青[5] Zhang Tao Cai Feng Cui He Ma Jiping Zhu Qianlin Hu Dongqing(Qingdao Technological University ,Qingdao 266033, China Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, China Qingdao Sunny HengPinglnstrument Co. Ltd , Qingdao 266109, China Ocean University of China, Qingdao 266100, China Qingdao Entry-Exit Inspection and Quarantine Bureau, Qingdao 266000, China)

机构地区：[1]青岛理工大学,青岛266033 [2]山东出入境检验检疫局,青岛266002 [3]青岛舜宇恒平仪器有限公司,青岛266109 [4]中国海洋大学,青岛266100 [5]青岛出入境检验检疫局,青岛266000

出　　处：《分析仪器》2017年第5期20-25,共6页Analytical Instrumentation

基　　金：国家重大科学仪器设备开发专项(2012YQ090229);青岛市民生计划项目(13-1-3-138-jh);山东省科技发展计划(2011GFS12005)

摘　　要：建立了离子色谱-质谱联用技术测定咖啡豆中阿拉伯糖、半乳糖、葡萄糖、蔗糖、木糖、甘露糖、果糖、核糖的分析方法。将样品粉碎后加入去离子水超声提取,经Carbopac^(TM)PA20(3*150mm)色谱柱分离,以EGC(自动淋洗液发生器)在线产生KOH为淋洗液,梯度淋洗,流速0.38mL/min,抑制器采用外加水模式,采用柱后补液法(Na^+源),串联质谱进行检测。结果表明,在一定的质量浓度范围内,上述单糖和小分子寡糖的色谱峰面积与质量浓度呈线性相关,线性相关系数(r)均大于0.999,样品的加标回收率均落在88.0~97.7%范围内,相对标准偏差均在2.36~3.70%之间(n=6)。该方法充分解决了含碳量不同的糖之间的假阳性问题,方法简便、快速、准确,可推广应用于咖啡豆中典型单糖和小分子寡糖的同时测定。A method was established for determination of arabinose, galactose, glucose, sucrose, xylose, mannose, fructose and ribose in coffee beans by using ion chromatography-electrospray tandem mass spectrometry in a positive ESI mode. Samples were separated on CarbopacTM PA20（3 × 150mm）analysis column by gradient elution with KOH eluent generated from an eluent generator cartridge（EGC）. The flow rate was 0.38mL/min and the post-column rehydration way（Na＋ source） was adopted. Under the optimal conditions, the linear correlation coefficient was better than 0. 999. The recoveries were in the range of 88.0% -97.7% while RSD values were between 2.36%-3.70%（n=6）. This method is sample,rapid and suitable for simultaneous determination of sugars in coffee beans.

关 键 词：离子色谱-质谱( IC-MS) 咖啡豆 单糖 寡糖 

分 类 号：O657.63[理学—分析化学] TS273[理学—化学]