C3d在人体正常肝Glisson囊的表达：一种非免疫性/渗透性沉积  

Expression of C3d in normal human liver tissues with non-immunologic osmostic mechanism

作　　者：黄海建[1] 冯昌银[2] 郑智勇 Huang Haijian Feng Changyin Zheng Zhiyong(Department of Pathology, Fujian Provincial Hospital, Fuzhou 350001, China)

机构地区：[1]福建医科大学省立临床医学院福建省立医院病理科,福州350001 [2]福建医科大学附属协和医院病理科 [3]福建医科大学福总临床医学院解放军福州总医院病理科

出　　处：《中华病理学杂志》2017年第9期629-633,共5页Chinese Journal of Pathology

基　　金：南京军区医药基金面上课题（08MA103）

摘　　要：目的 探讨正常人肝汇管区Glisson囊中C3d沉积形成的原因和意义.方法 采用免疫组织化学方法对60例人体各年龄段的正常人肝组织标本进行补体与抗体标记,同时对部分标本进行C3d胶体金免疫电镜染色,其他器官及组织95例的C3d染色作为对照观察.结果 60例正常肝组织中50例（50/60,83.3%）见C3d沉积,主要见于Glisson囊,其次小动脉内膜、肝静脉外膜也有不同程度C3d沉积.C3d表达于20岁以上正常人较多,20岁及以下正常人次之,3岁及以下正常人无表达.免疫电镜显示C3d胶体金颗粒疏密不均地沉积于Glisson囊胶原纤维之间,无免疫沉积物伴随.40例脾脏组织中30例（30/40,75.0%）见C3d沉积,为脾包膜纤维层、脾小梁纤维鞘、小梁动脉和中央动脉的内膜.结论 C3d沉积于〉3岁正常人的肝Glisson囊、脾小梁纤维鞘及肝脾小动脉内膜,是一种非免疫性、血浆渗出性沉积,属于正常的生理现象.应避免将这种沉积视为免疫复合物沉积或者体液免疫反应现象.Objective To observe the deposition of complement C3d at different development stages in human normal organs and tissues, and investigate the significance of its deposition.Methods Using immunohistochemical methods, the deposition of C3d was detected at different development stages of 60 normal human organs and tissue specimens and double staining was performed in some specimens.Ninty-five cases of other organs or tissues were collected as control group.Results In 50 of 60 livers, it was observed the deposition of C3d in Glisson＇s capsule and periportal sheath, with irregular linear network-like disposition surrounding the portal sheath.In different age groups, the expression of C3d was more beyond the 20 year-old group than 3 to 20 year-old group.There wasn＇t any expression of C3d under 3-year-old group.Under the immuning electron micrograph, C3d depositing at the Glisson＇s capsule was observed, without immuning compounding.Thirty in 40 spleens, deposition of C3d in capsules, arteries of lymphatic sheath, follicles in the spleen was observed.Conclusions The deposition of C3d in Glisson＇s capsule, splenic trabeculae, fibrous sheath, endarterium of liver and spleen arterioles, within normal human tissues from patients elder than 3 years, are osmosis/immunogenic deposition.The deposition of C3d is a normal physiological phenomenon, and treatment of the deposition of C3d should be avoided, as it is an immune complex or immuning reaction phenomenon.

关 键 词：肝 脾 补体C3d 免疫组织化学 

分 类 号：R575[医药卫生—消化系统]