结肠癌microRNA表达谱检测及生物信息学分析  

Expression profile of micro RNAs and bioinformatics analysis in human colon cancer tissues

作　　者：黄幼生[1] 翁阳[1] 解娜[1] 张艺馨[1] 罗志飞[1] 薛逢贵 

机构地区：[1]海南医学院第一附属医院病理科,海南海口570102

出　　处：《海南医学》2017年第18期2928-2931,共4页Hainan Medical Journal

基　　金：国家自然科学基金(编号:81260321)

摘　　要：目的探讨miRNA(micro RNA,微小RNA)在结肠癌组织中的表达特征及生物学功能。方法选取临床特征相似的3对结肠癌组织及其配对正常黏膜组织,应用Exiqon miRNA芯片检测miRNA在结肠癌及其配对组织中差异表达情况;3个差异表达的miRNA被选取进行q PCR验证;生物信息学分析差异表达的miRNA及其靶向基因在结肠癌进展中的作用机制。结果芯片结果显示,在3对结肠癌组织中有201个miRNA出现上调表达,94个下调表达(差异倍数>2),差异有统计学意义(P<0.05)。qPCR结果显示,与对照组织比较,选取的3个miRNA中hsa-miR-18b-3p、hsa-miR-31-5p在结肠癌组织中表达上调,hsa-miR-142-3p表达下调,与芯片结果一致,差异有统计学意义(P<0.05)。GO及pathway分析显示差异表达的miRNA涉及结肠癌细胞的分化、迁移、定位、增生及RNA、蛋白合成等功能调控,与细胞粘附、结肠癌发生发展等信号途径的激活相关。结论结肠癌组织中miRNA存在异常表达,可能涉及结肠癌的发生、发展。Objective To investigate the expression profile and biological functions of miRNAs （microRNA） in human colon cancer tissues. Methods The expression profiles of miRNAs were compared between 3 pairs of colon cancer and its adjacent normal tissues using a Exiqon miRNA array, following which quantitative PCR （qPCR） was employed to confirm the results of the miRNA array, and 3 differentially expressed miRNAs were selected for qPCR validation. Bioinformatics was used to analyze the biological function of the differentially expression miRNAs and its target genes in colon cancer. Results Compared with adjacent normal mucosal tissues, 201 miRNAs were up-regulated and 94 miRNAs were down-regulated in colon cancer tissues （fold〉2）, the difference was statistically significant （P〈0.05）. The qPCR results showed that, compared with the control group, the expression of hsa-miR-18b-3p and hsa-miR-31-5p were up-regulated and the expression of hsa-miR-142-3p was down regulated in colon cancer tissues, which were con- sistent with microarray-based expression analysis, with statistically significant difference （P〈0.05）. Furthermore, the online GO and KEGG pathwany analysis revealed that the differentially expressed miKNAs may be involving cell differentiation, migration, localization, proliferation, synthesis of RNA and protein and other biological functions, and perhaps related to cell adhesion and activation of signaling pathways of colon cancer development. Conclusion There are abnormal expression of miRNAs in colon cancer tissues, which may be related to colon cancer tumorigenesis.

关 键 词：MIRNA 结肠癌 差异表达 生物信息学 MIRNA芯片 

分 类 号：R735.35[医药卫生—肿瘤]