胁迫中华蜜蜂幼虫肠道的球囊菌及其体外培养的高表达基因分析  

Analysis of highly expressed genes of Ascosphaera apis infecting the gut of Apis cerana cerana larvae and its in vitro culture

作　　者：陈大福[1] 王鸿权 李汶东 熊翠玲[1] 郑燕珍[1] 付中民[1] 徐细建[1] 黄枳腱 郭睿[1] 

机构地区：[1]福建农林大学蜂学学院,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2017年第5期562-568,共7页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：现代农业产业技术体系建设专项资金(CARS-44-KXJ7);福建省中青年教师教育科研项目(JAT17018);福建农林大学科技发展基金(KF2015123);国家级大学生创新创业项目(201610389016)

摘　　要：利用RNA-seq技术对胁迫中华蜜蜂(简称中蜂)6日龄幼虫肠道的球囊菌及其体外培养进行深度测序,根据基因的FPKM值筛选得到高表达基因(HEGs),进而对HEGs进行GO及KEGG代谢通路(pathway)富集分析.Illumina测序数据经质控和过滤得到100814558条有效读段(clean reads),平均Q30均在93.81%以上.GO富集分析结果显示处理组(Aac T)的HEGs富集于39个GO term,基因富集数最多的是细胞(1872 unigene)、细胞组件(1872 unigene)和细胞进程(1748 unigenes);对照组(Aa CK)的HEGs富集于37个GO term,基因富集数最多的是细胞(785 unigenes),其次是细胞组件(785 unigenes)和代谢进程(776 unigenes).KEGG pathway富集分析显示,Aac T的HEGs富集在119个代谢通路上,基因富集数最多的是核糖体(176 unigenes)、碳代谢(147 unigenes)及氨基酸的生物合成(134 unigenes);Aa CK的HEGs富集在110个代谢通路上,基因富集数最多的是核糖体(179 unigenes)、氨基酸的生物合成(70 unigenes)以及碳代谢(62 unigenes).深入分析发现,对于富集在MAPK信号通路上的高表达基因数量,胁迫中蜂幼虫肠道的球囊菌远多于体外培养的球囊菌,说明病原的该通路在胁迫后期被显著激活.In this study, Ascosphaera apis spores （AacT）, from the gut of 6-day-old Apis cerana cerana larvae, and A.c.cerana were sequenced utilizing RNA-seq technology. Pure A. apis spore was used as the control （ AaCK） . A total of 100814558 clean reads with a mean Q30 of 93.81% were obtained after quality control and filtration. GO enrichment analysis suggested that the highly ex-pressed genes （ HEGs） in AacT were involved in 39 terms, which was mostly enriched in cell （ 1872 unigenes） , followed by cellular component （ 1872 unigenes） and cellular process （ 1748 unigenes） . And the HEGs in AaCK were engaged in 37 terms, with the lar-gest group in cell （ 785 unigenes） , followed by cellular component （ 785 unigenes） and metabolic process （ 776 unigenes） . KEGG enrichment analysis showed that the HEGs in AacT were enriched in 119 pathways, dominating in ribosme （176 unigenes）, fol-lowed by carbon metabolism （147 unigenes） and biosynthesis of amino acids （134 unigenes）. While the HEGs in AaCK were in-volved in 119 pathways, and the largest groups were ribosome （ 179 unigenes） , biosynthesis of amino acids （ 70 unigenes） and car-bon metabolism （ 62 unigenes） .

关 键 词：中华蜜蜂 RNA-SEQ 球囊菌 高表达基因 

分 类 号：S895.1[农业科学—特种经济动物饲养]