重组人红细胞生成素对新型支气管肺发育不良早产鼠模型血管内皮生长因子表达的影响  

Effects of recombinant human erythropoietin on the expression of vascular endothelial growth factor in premature rat model of the new type bronchopulmonary dysplasia

作　　者：赵日红[1] 白纪红[2] 刘曼[1] 黄艳 

机构地区：[1]桂林医学院附属医院新生儿科,广西桂林541000 [2]桂林医学院附属医院研究生科,广西桂林541000 [3]桂林医学院附属医院教学管理部,广西桂林541000

出　　处：《重庆医学》2017年第29期4040-4043,共4页Chongqing medicine

基　　金：广西高校科学技术研究基金资助项目(KY2015LX254);广西自然科学基金资助项目(2014GXNSFAA118152)

摘　　要：目的观察重组人红细胞生成素(rhEPO)对新型支气管肺发育不良(BPD)早产鼠模型肺组织血管内皮生长因子(VEGF)蛋白及mRNA表达的影响。方法60只定期受孕SD大鼠于孕第15天孕囊内注射脂多糖(LPS)或磷酸盐缓冲液(PBS),孕第21天剖宫娩出早产鼠,早产鼠于生后6h内分为5组:PBS+空气组、PBS+高氧组、LPS+高氧组、PBS+高氧+rhEPO组、LPS+高氧+rhEPO组。rhEPO(1 200IU/kg)干预组于高氧暴露6h后开始第1次背部皮下注射,隔天1次,共7次。分别在高氧暴露第1、7、14天处死早产鼠,观察各组生存率、体质量、肺质量并计算肺质量/体质量比值,观察肺组织病理变化,采用蛋白质印迹法(Western blot)和反转录PCR(RT-PCR)法检测肺组织VEGF蛋白及mRNA表达水平。结果与PBS+空气组比较,经高氧干预后早产鼠生存率降低、体质量减轻、肺质量增加,病理损伤加重,肺组织VEGF蛋白及mRNA表达水平降低,并且以LPS+高氧组更明显(P<0.05),肺质量/体质量比值增加(P>0.05);rhEPO干预后上述指标得到相应改善(P<0.05)。结论 rhEPO可能通过参与调解VEGF相关通路,对BPD模型早产鼠起到一定的干预和治疗作用,提高了生存率。Objective To investigate the effects of recombinant human erythropoietin （rhEPO） on the expression of vascular endothelial growth factor （VEGF） protein and mRNA in lung tissue of premature rat model of the new type bronchopulmonary dysplasia （BPD）. Methods Lipopolysaccharide （LPS） or PBS was injected into 60 pregnant rats on the 15th day of gestation. The premature rats by cesarean delivery on the 21th day of gestation were divided into 5 groups： PBS ＋air group, PBS ＋ hyperoxia group, LPS＋ hyperoxia group, PBS＋ hyperoxia ＋ rhEPO group, LPS＋ hyperoxia ＋ rhEPO group. In rhEPO intervention groups, after 6 h exposure to hyperoxia,rhEPO （1 200 IU/kg） was administrated subcutaneously,once every other day for 7 times. The survival rate, body weight,lung weight and lung weight/body weight ratio and pathological changes of lung tissue were obseryed, the expression levels of VEGF protein and mRNA in the lung tissue were determined by Western blot and RT-PCR at the l st,7th and 14th day after hyperoxia exposure. Results Compared with the PBS＋air group,the survival rate and body weight were decreased, the lung weight was increased, the lung pathological damages were more serious,the expression levels of VEGF protein and mRNA in lung tissue were decreased after hyperoxia exposure. These changes were more notable in the LPS＋hyperoxia group （P〈0.05）. The lung weight/body weight ratio showed an increasing tendency （P〈0.05）. The above indexes were improved after rhEPO intervention （P〈0.05）. Conclusion rhEPO could increase the survival rate and produce certain intervention and treatment effects by regulating the VEGF relative pathway in BPD model rats.

关 键 词：支气管肺发育不良 红细胞生成素 重组 血管内皮生长因子 

分 类 号：R-332[医药卫生] R722.6