超声作用下2种压电陶瓷多孔支架材料上成骨细胞的增殖与分化  

Effect of Low-intensity Pulsed Ultrasound on the Proliferation and Differentiation of Osteoblasts Cultured on Two Piezoelectric Ceramic Porous Scaffolds

作　　者：全泉 矫义来[2] 曹洪娟 吴琳[1] 

机构地区：[1]中国医科大学附属口腔医院修复一科,沈阳110002 [2]中国科学院金属研究所材料特种制备与加工研究部,沈阳110016

出　　处：《中国医科大学学报》2017年第10期869-873,共5页Journal of China Medical University

基　　金：国家高技术研究发展计划(863计划)(2015AA033702);辽宁省自然科学基金(2014021008)

摘　　要：目的在低强度脉冲超声波(LIPUS)作用下对比研究钛酸钡/微弧氧化钛合金多孔材料(简称BaTiO_3)和氧化锌/微弧氧化钛合金多孔材料(简称ZnO)的表面MC3T3-E1成骨细胞增殖和分化的特性。方法分别将BaTiO_3和ZnO这2种材料随机分成超声组(UBaTiO_3组和UZnO组)和对照组(CBaTiO_3组和CZnO组),将MC3T3-E1成骨细胞与材料复合培养,超声组每天接受20 min LIPUS辐照,对照组为不开功率源假辐照。通过检测噻唑兰(MTT)、碱性磷酸酶(ALP)和骨钙素(OCN)值观察成骨细胞增殖和分化的水平。结果 4 d时UBaTiO_3组MTT值高于CBaTiO_3组,差异有统计学意义(P<0.05);1、4和7 d时UBaTiO_3组和CBaTiO_3组的MTT值均高于UZnO组和CZnO组,差异均有统计学意义(P<0.05)。4和7 d时UBaTiO_3组的ALP值高于CBaTiO_3组,7 d时UZnO组的ALP值高于CZnO组,差异均有统计学意义(P<0.05);4和7 d时UBaTiO_3组和CBaTiO_3组的ALP值均高于UZnO组和CZnO组,差异均有统计学意义(P<0.05)。7、10和14 d时UBaTiO_3组的OCN值高于CBaTiO_3组,14 d时UZnO组的OCN值高于CZnO组,差异均有统计学意义(P<0.05),7、10和14 d时UBaTiO_3组的OCN值高于UZnO组,10和14 d时CBaTiO_3组的OCN值高于CZnO组,差异均有统计学意义(P<0.05)。结论 LIPUS可以促进BaTiO_3和ZnO这2种材料上MC3T3-E1的增殖与分化。在LIPUS作用下,BaTiO_3材料优于ZnO材料,更具有成为骨修复材料的潜能。Objective To evaluate the effects of low-intensity pulsed ultrasound （LIPUS） treatment on the proliferation and differentia- tion of osteoblasts cultured on barium titanate/titanium alloy （BaTiO3） and zinc oxide/titanium alloy （ZnO） piezoelectric ceramic composite scaffolds. Methods The BaTiO3 and ZnO porous composite scaffolds were randomly divided into ultrasound （UBaTiO3 and UZnO） and control （CBaTiO3 and CZnO） groups, and MC3T3-E1 cells were cultured on the surface of the materials in each group. Cells in the ultrasound groups were subjected to a 20-min treatment LIPUS session daily, and the control group was sham-treated without the power source. The proliferation and differentiation rates of osteoblasts were evaluated using methyl thiazolyl tetrazolium （MTT）, alkaline phosphatase （ALP）, and osteocalcin （OCN） assays. Results Significant differences in the MTT values were observed between the UBaTiO3 and CBa- TiO3 groups at day 4 （P 〈 0.05） and between the UBaTiO3 and UZnO groups at days 1,4,and 7 （P 〈 0.05）. Compared with the CBaTiO3 group,the UBaTiO3 group showed significantly higher ALP activity and OCN levels at days 4 and 7 （P 〈 0.05）. Further,a significant difference in the ALP and OCN values between the UBaTiO3 and UZnO groups （P 〈 0.05） was observed. Conclusion LIPUS promotes the proliferation and differentiation of osteoblasts on BaTiO3 and ZnO scaffolds. BaTiO3 scaffolds enable better promotion of osteogenesis under LIPUS treatment than ZnO scaffolds do.

关 键 词：低强度脉冲超声波 成骨细胞 压电陶瓷 压电效应 

分 类 号：R783.1[医药卫生—口腔医学]