白藜芦醇对缺氧诱导的肝癌细胞株BEL-7402增殖及凋亡的影响  

Restorative in Hepatocellular Carcinoma Cell Line BEL-7402 Growth and Apoptosis during Hypoxia

作　　者：周婷[1] 韩继武[1] 刘铁夫[1] 孟宪红[1] 田佳[1] 

机构地区：[1]哈尔滨医科大学附属第四医院,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2017年第28期5412-5415,5420,共5页Progress in Modern Biomedicine

摘　　要：目的:探讨不同浓度白藜芦醇在常氧环境及缺氧环境下对肝癌细胞株BEL-7402增殖及凋亡的影响。方法:采用MTT法分析不同浓度(0、12.5、25、50、100、200μmol/L)白藜芦醇在常氧环境及缺氧环境下对肝癌细胞株BEL-7402增殖的影响,应用流式细胞仪检测其对BEL-7402细胞凋亡的影响。结果:不同浓度的白藜芦醇在常氧及缺氧环境中对肝癌细胞株BEL-7402均有明显抑制增殖及诱导凋亡作用,呈剂量-效应相关,其中50、100、200μmol/L白藜芦醇两种环境中对肝癌细胞的抑制率及凋亡率与对照组比较差异均具有统计学意义(P<0.05)。50、100、200μmol/L白藜芦醇,常氧环境中对肝癌细胞的抑制率分别为18.70±2.15%、22.13±2.18%、31.47±2.26%,凋亡率分别为11.82±2.28%、19.80±2.64%、38.00±2.57%;缺氧环境中对肝癌细胞的抑制率分别为17.87±1.76%、23.52±2.29%、29.87±1.51%,凋亡率分别为12.36±3.15%、19.71±2.78%、37.87±4.83%。结论:白藜芦醇在缺氧环境下能抑制肝癌细胞株BEL-7402的增殖并诱导其凋亡,且其作用与常氧环境相似。Objective： Explore the different concentration of resveratrol in hepatocellular carcinoma cell lines under the condition of oxygen and hypoxia BEL - 7402 growth and apoptosis. Methods： Determined by MTT method, analysis of different concentrations （0, 12.5, 25, 50, 100, 200 μmol/L） resveratrol under hypoxia and oxygen environment influence on hepatocellular carcinoma cell line BEL-7402 proliferation, loss of cell is used to test the effect of BEL-7402 cell apoptosis. Results： Different concentration ofresveratrol in oxygen and hypoxia environment to hepatoeellular carcinoma cell line BEL - 7402 all have significantly inhibit proliferation and induce apoptosis, the dose - effect, including 50, 100, 200 μmol/L resveratrol two environment on hepatocellular carcinoma cell inhibition rate and apoptosis rate were compared with controls （P 〈 0. 05）, with statistical significance.50, 100, 200 μmol/L resveratrol, oxygen environment inhibition rate of hepatocellular carcinoma cells were 18.70± 2.15%, 22.13 ± 2.18% and 31.47± 2.26%, apoptosis rate were 11.82± 2.28%, 19.80± 2.64% and 38.00± 2.57%; Hypoxia environment on hepatocellular carcinoma cancer cell inhibition rate were 17.87± 1.76%, 23.52± 2.29%, 29.87± 1.51%, apoptosis rote were 12.36± 3.15%, 19.71 ± 2.78%, 37.87± 4.83%. Conclusion： Resveratrol in hypoxia environment can inhibit hepatocellular carcinoma cell line BEL - 7402 proliferation and induce its apoptosis, and its action is similar to normal oxygen environment.

关 键 词：白藜芦醇 缺氧 肝癌 

分 类 号：R735.7[医药卫生—肿瘤]