tBHQ和SFN在创伤性脑损伤小鼠中的疗效比较性分析  

Comparative Analysis of Therapeutic Effect between tBHQ and SFN in Rats with Traumatic Brain Injury

作　　者：刘晓斌[1] 侯明山[1] 李民[1] 孟发财[1] 黄卫东[1] 宋锦宁[2] 

机构地区：[1]陕西省人民医院神经外科,陕西西安710004 [2]西安交通大学第一附属医院神经外科,陕西西安710004

出　　处：《现代生物医学进展》2017年第28期5445-5448,共4页Progress in Modern Biomedicine

摘　　要：目的:探讨叔丁基对苯二酚(t BHQ)和莱菔硫烷(SFN)在患有创伤性脑损伤大鼠的疗效差异性。方法:80只健康成年的雄性SD大鼠分为假手术组、常规损伤组、t BHQ治疗组和SFN治疗组,使用电子颅脑损伤仪(e CCI)制备TBI模型。其中t BHQ治疗组在伤前24 h大鼠腹腔注射三次t BHQ(50 mg/kg),每8 h一次;SFN治疗组在伤后15 min给予腹腔注射SFN(5 mg/kg)。给药24 h后,采用m NSS方法评价各组大鼠神经功能缺损状况,利用干湿称量法计算脑含水量,Western blot和ELISA方法分别测定大鼠脑组织的NOX2和Nrf2的表达水平。结果:损伤发生后第24 h,t BHQ治疗组和SFN治疗组在m NSS评分((4.5±0.71)vs(9.2±0.79),(6.0±0.82)vs(9.2±0.79))、脑水肿(79.4%vs 85.6%,80.3%vs 85.6%)、NOX2和Nrf2(0.93 ng/m L vs 0.81 ng/m L,0.87 ng/m L vs 0.81 ng/m L)表达上与常规损伤组差异明显,而t BHQ治疗组和SFN治疗组间在m NSS评分((4.5±0.71)vs(6.0±0.82))、NOX2和Nrf2(0.93 ng/m L vs 0.87 ng/m L)表达上差异显著。结论:在大鼠TBI模型中,t BHQ和SFN均可以有效的降低机体自身的氧化应激作用,并改善神经功能,但t BHQ的疗效要好于SFN。Objective： To explore the difference of therapeutic effect between tert-butylhydroquinone （tBHQ） and sulforaphane （SFN） in rats with traumatic brain injury. Methods： A total of 80 healthy adult male rats were randomly divided into 4 groups： sham group, TBI group, tBHQ treatment group and SFN treatment group, then the traumatic brain injury （TBI） model were prepared by eCCI. tBHQ treatment group rats were given intraperitoneal injection three times tBHQ （50 mg/kg） before injury 24 h, and SFN treatment group rats were given intraperitoneaUy injected SFN （5 mg/kg） after injury 15 rain. Neurologic deficits was evaluated 24 h after TBI by mNSS method, brain edema was detected by the wet-dry ratio method. The expression levels of NOX2 protein and Nrt2 protein in the brain tissue were investigated using Western blot and ELISA analysised, respectively. Results： Compared with TBI group, there were significant difference on mNSS scores （（4.5± 0.71） vs （9.2± 0.79）, （6.0± 0.82） vs （9.2± 0.79））, brain edema （79.4% vs 85.6%, 80.3% vs 85.6%）, the expression levels of NOX2 protein and Nrf2 protein （0.93 ng/mL vs 0.81 ng/mL, 0.87 ng/mL vs 0.81 ng/mL） in tBHQ treatment group and SFN treatment group. In addition, there were also significant differences on mNSS scores （（4.5± 0.71） vs （6.0± 0.82））, the expression levels of NOX2 protein and Nrf2 protein （0.93 ng/mL vs 0.87 ng/mL） between tBHQ treatment group and SFN treatment group. Conclusion： In the TBI model of rats, tBHQ and SFN could effectively attenuated cerebral oxidative stress and improved nerve fimction after TBI, but the curative effect oftBHQ was better than the SFN.

关 键 词：叔丁基对苯二酚 菜菔硫烷 脑损伤 Nd2 NOX2 

分 类 号：R-33[医药卫生] R651.1