香茅醇微乳凝胶的制备及体外抗菌试验  

Preparation and in Vitro Antibacterial Effect of Citronellol Microemulsion Gel

作　　者：姜丰[1] 王益[1] 严俊丽[1] 江滟[1] 周雪[1] 沈祥春[1] 陶玲[1] 

机构地区：[1]贵州医科大学贵州省普通高等学校天然药物药理与成药性评价重点实验室,贵阳550025

出　　处：《中国实验方剂学杂志》2017年第20期8-13,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省高等教育科技创新团队项目(黔教合人才团队字[2014]31);贵州省高层次创新型人才百层次人才项目(贵州科技厅黔科合人才[2015]4029号);贵州省科技创新人才团队项目(黔科合人才团队[2015]4025号);贵州省教育厅拔尖人才计划项目(黔教合KY2016[073])

摘　　要：目的:优选香茅醇微乳凝胶的处方及制备方法并对其进行体外抗菌试验研究。方法:以微乳区域面积百分比为指标,使用伪三元相图法优化香茅醇微乳的处方;以微乳凝胶的外观、黏稠度、延展性、油腻性为指标,通过单因素试验及正交试验优化香茅醇微乳凝胶的处方工艺。采用试管二倍稀释法考察香茅醇微乳凝胶对金黄色葡萄球菌及白色念珠菌的最低抑菌浓度(MIC)和最小杀菌浓度(MBC),通过时间-杀菌效率试验考察香茅醇微乳凝胶及香茅醇微乳的杀菌效率。结果:香茅醇微乳凝胶的最佳处方是15-羟基硬脂酸聚乙二醇酯-乙醇-香茅醇-卡波姆-980-甘油-水(0.518∶1.037∶0.667∶0.8∶5∶91.978)。香茅醇微乳为淡蓝色透明均一液体,平均粒径54.9 nm,Zeta电位^(-1)0.22 m V。香茅醇微乳凝胶对金黄色葡萄球菌及白色念珠菌的MIC和MBC均为1.667 g·L^(-1);时间-杀菌效率试验结果表明香茅醇微乳凝胶及香茅醇微乳在2 h对受试菌种的杀菌率达100%。结论:优选的香茅醇微乳凝胶处方工艺稳定可行,该制剂对金黄色葡萄球菌和白色念珠菌均有杀伤作用,且起效快。Objective： To optimize preparation technology of citronellol microemulsion gel and study on its in vitro antibacterial property. Method： The prescription of citronellol microemulsion was optimized by pseudoternary phase diagram with the percentage of microemulsion area as index. Single factor tests and orthogonal test were adopted to optimize formulation technology of citronellol microemulsion gel with appearance, viscosity,ductility,greasiness as indexes. Tube double dilution method was used to investigate minimum inhibitory concentration（ MIC） and minimum bactericidal concentration（ MBC） of citronellol microemulsion gel to the tested bacteria. The bactericidal efficiency of citronellol microemulsion gel and citronellol microemulsion were tested by the experiment of time-sterilization efficiency. Result： Optimum prescription of citronellol microemulsion gel was as follows： citronellol of 0. 518%,Kolliphor HS 15 of 1. 037%,ethanol of 0. 667%,carbomer-980 of0. 8%,glycerol of 5%,water of 91. 978%. The acquired microemulsion exhibited nattier blue,it was uniform and transparent with the average particle size of 54. 9 nm and Zeta potential of^（-1）0. 22 m V. The MIC and MBC of citronellol microemulsion gel were 1. 667 g·L^（-1） for the tested bacteria（ Staphylococcus aureus and Candida albicans）. Time-sterilization efficiency indicated sterilizing rates were 100% of citronellol microemulsion gel to the tested bacteria at 2 h. Conclusion： This optimized formulation technology of citronellol microemulsion gel is stable and feasible,this preparation has a rapid bactericidal effect on S. aureus and C. albicans.

关 键 词：香茅醇 微乳 凝胶 处方工艺 体外抗菌试验 伪三元相图法 试管二倍稀释法 

分 类 号：R283.6[医药卫生—中药学] R945[医药卫生—中医学]