黄芪多糖的脱蛋白工艺考察  

Investigation of Deproteinization of Astragalus Polysaccharide

作　　者：胡媛媛[1] 吕瑞红[1] 纪永升[1] 褚意新[1] 杜志谦[1,2] 

机构地区：[1]河南中医药大学,郑州450046 [2]洛阳正骨医院河南省骨科医院,郑州450046

出　　处：《中国实验方剂学杂志》2017年第20期25-29,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金青年基金项目(21505034);河南中医学院省属高校科研专项(2014KYYWF-QN15);河南中医学院科技创新人才项目(2016XCXRC02);郑州市科技攻关项目(20150305)

摘　　要：目的:优选黄芪多糖的脱蛋白工艺,提高黄芪多糖的纯度,为该有效部位的后续药理研究奠定基础。方法:采用水提醇沉法提取黄芪多糖,以黄芪多糖的含量和蛋白质的脱除率为考察指标,在单因素试验基础上,通过正交试验考察酶解时间、酶底比、酶解液p H和酶解温度对黄芪多糖脱蛋白工艺的影响,利用酶-Sevage法除蛋白。结果:最佳脱蛋白工艺为酶底比2.0%,p H 5.0,50℃水浴酶解24 h;在该工艺条件下,蛋白脱除率87.80%,黄芪多糖质量分数83.47%。结论:优选的脱蛋白工艺条件稳定可行,酶-Sevage法可显著提高黄芪多糖的纯度。Objective： Astragalus polysaccharide is an important active ingredient with the immune regulation,anti-oxidation,anti-cancer,anti-atherosclerosis and so on. To remove the proteins from astragalus polysaccharide is a critical procedure,because the content of protein in astragalus polysaccharide was higher than15%. In this work,the purity would be improved by removing the proteins from astragalus polysaccharide,which can be in favor of the study on pharmacological activity and mechanisms of this effective site. Method： Astragalus polysaccharide was prepared by the water-extraction and alcohol-precipitation method, and the proteins in astragalus polysaccharide were removed by enzymatic-Sevage process, then the technolology was optimized with orthogonal test and single factor tests, respectively. Optimum technology was selected based on the purity of astragalus polysaccharide and the content of proteins. Result： Optimum process of deproteinization was as follows：the enzyme-substrate ratio of 2. 0%, p H of 5. 0, hydrolysis for 24 h at 50 ℃; under these conditions, the removal rate of proteins and the content of astragalus polysaccharide were 87. 80% and 83. 47%,respectively. Conclusion： This optimal technology is stable and feasible, enzymatic-Sevage method can significantly improve the purity of astragalus polysaccharide.

关 键 词：黄芪 多糖 脱蛋白 酶-Sevage法 工艺优化 

分 类 号：R283.6[医药卫生—中药学] R945[医药卫生—中医学]