明胶-溶菌酶/ZnO纳米复合抗菌膜材料构建与性能  

Fabrication and Characterization of Gelatin-Lysozyme/Nano-Zno Nanocomposite Films

作　　者：胡二坤[1] 石伟健 尹寿伟 

机构地区：[1]河南职业技术学院烹饪食品系,河南郑州450046 [2]华南理工大学食品科学与工程学院食物蛋白工程研究中心,广东广州510640

出　　处：《现代食品科技》2017年第9期159-165,共7页Modern Food Science and Technology

基　　金：国家自然科学基金(31471628);华南理工大学中央高校基本科研业务费重点项目(2017ZD080);华南理工大学制浆造纸工程国家重点实验室(201536)

摘　　要：本研究以温敏性的明胶为基材同时包埋有机/无机抗菌剂,企图实现抗菌剂的协同增效,扩大材料的抗菌谱,并系统研究抗菌材料表面结构、机械与阻隔性能随抗菌剂种类及计量的演变规律。溶菌酶/纳米氧化锌-明胶膜材料具有选择吸光性,在紫外区域有明显吸收峰,而在可见光区域透光率超过90%。溶菌酶/纳米氧化锌具有协同增效,扩大膜材料的抗菌谱,相对荷载溶菌酶或纳米氧化锌的膜材料,复合抗菌剂膜对大肠杆菌与枯草芽孢杆菌具有更强的抗菌效果。荷载溶菌酶或纳米氧化锌不改变明胶膜材料的表面微结构,两者在成膜及膜基质中的相互作用形成异质的膜表面,并影响蛋白网络结构,弱化膜材料的水汽及氧气阻隔性能。本研究为新型有机/无机复合抗菌膜材料开发提供理论依据。This work attempted to achieve effective antibacterial gelatin films by incorporating organic / inorganic antibacterials leading to synergistic antibacterial effect and wide antibacterial spectrum. Surface morphology, mechanical and barrier properties as well as antimicrobial activity of the films were evaluated. Gelatin-lysozyme/nano-ZnO nanocomposite films had selective light absorbance. Obvious absorption peak occurred in the ultraviolet （UV） region, while the transmittance was over 90% in the visible region. The simultaneous incorporation of lysozyme / nano-ZnO resulted in synergistic antibacterial activity against test organism, i.e., Escherichia coli （G-） and Bacillus subtilis （G＋）. The loading of lysozyme or nano ZnO within the gelatin matrix did not affect the surface morphology of the films, while the simultaneous loading of lysozyme and nano-ZnO resulted in heterogeneous surface morphology, probably due to the formation of the agglomerates via the interaction between lysozyme and nano-ZnO in the filim-forming solution or the casting process. As a result, gelatin network structure as well as the performance of water vapor and oxygen barrier was varied, in an antimicrobial type-and dose- dependent manner. This study provides a theoretical basis for the development of novel organic/inorganic composite films.

关 键 词：抗菌膜 明胶 溶菌酶 纳米氧化锌 协调抗菌 

分 类 号：TS206.4[轻工技术与工程—食品科学]