分散固相萃取-气相色谱法测定含油食品中亚胺唑的残留  

Determination of imibenconazole in fat-containing foods by dispersive solid phase extraction-gas chromatography

作　　者：张文君[1] 李慧冬[1] 方丽萍[1] 董崭[1] 杜红霞[1] 官帅 陈子雷[1] 

机构地区：[1]山东省农业科学院农业质量标准与检测技术研究所,济南250100

出　　处：《农药学学报》2017年第5期609-616,共8页Chinese Journal of Pesticide Science

基　　金：山东省农业科学院重大科技成果培育计划(2015CGPY04)

摘　　要：建立了检测含油类食品(核桃、花生油和干辣椒粉)中亚胺唑残留的分散固相萃取-气相色谱分析方法。样品中的亚胺唑经乙腈提取,N-丙基乙二胺吸附剂(PSA)和十八烷基键合硅胶吸附剂(C18)混合分散固相萃取净化剂净化,气相色谱法测定,基质标准曲线-外标法定量。结果显示:在0.005~10 mg/L范围内,亚胺唑的色谱峰面积与其质量浓度间呈良好的线性关系,相关系数r>0.99;定量限为0.05 mg/kg。在0.05、0.1和1 mg/kg添加水平下,亚胺唑在核桃、花生油及干辣椒粉基质中的添加回收率为82%~107%,相对标准偏差为3.0%~6.3%。方法各项性能均能满足对含油食品中亚胺唑残留分析的要求。A new method for detecting imibenconazole in fat-containing foods （walnut, peanut oil and red chilli powder） using gas chromatograph coupled with dispersive solid-phase extraction was developed. Imibenconazole in samples were extracted by acetonitrile and purified by PSA and C18. it was then qualitatively and quantitatively determined by GC with matrix standard curve-external standard method. The results showed that the good linearity has been achieved between chromatographic peak and concentration of imibenconazole in the range of 0.005-10 mg/L, the correlation coefficient r was greater than 0.99, and the limit of quantification （LOQ） was 0.05 mg/kg. At spiked levels of 0.05, 0.1, and 1 mg/kg, recoveries of imibenconazole in walnut, peanut oil and red chilli powder were 82%-107% and relative standard deviations were 3.0%-6.3%. Therefore, this method is suitable for the determination of imibenconazole in fat-containing foods.

关 键 词：分散固相萃取 气相色谱 含油食品 亚胺唑 基质效应 

分 类 号：O657.71[理学—分析化学] TQ450.263[理学—化学]