肝内胆管癌组织中GRP78和IL-6的表达  

Expressions of GRP78 and IL-6 in intrahepatic cholangiocarcinoma tissue

作　　者：唐文超[1] 翟文龙[1] 叶健文[1] 王刚[1] 李志远[1] 陈向东 

机构地区：[1]郑州大学第一附属医院肝胆胰外科,郑州450052

出　　处：《郑州大学学报（医学版）》2017年第5期609-613,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省高校科技创新人才支持计划14HASTIT030

摘　　要：目的:探讨葡萄糖调节蛋白78(GRP78)和白细胞介素-6(IL-6)在肝内胆管癌组织中的表达。方法:采用免疫组化法检测GRP78和IL-6在48例肝内胆管癌,48例肝内胆管结石及30例正常肝内胆管组织中的表达。结果:GRP78和IL-6在肝内胆管癌组织中的阳性表达率分别为64.6%和60.4%,在肝内胆管结石胆管组织中的阳性表达率分别为75.0%和68.8%,均高于正常肝内胆管组织(6.7%和13.3%)(P<0.05)。肝内胆管癌组织中GRP78和IL-6的表达呈正关联(rP=0.280,P=0.044)。GRP78阳性表达与癌组织的分化程度(P=0.035)、淋巴结转移(P=0.015)、TNM分期(P<0.01)有关;GRP78阳性表达与阴性表达患者相比,生存曲线左移(P<0.05),中位生存时间较短。IL-6阳性表达与癌组织的淋巴结转移(P=0.008)及TNM分期(P=0.015)有关;IL-6阳性表达与阴性表达患者比较,生存曲线差异无统计学意义(P=0.331)。结论:GRP78和IL-6可能参与了肝内胆管组织的恶变过程,并与胆管癌的生长、转移关系密切。Aim： To explore the expressions of GRP78 and IL-6 in intrahepatic cholangiocarinoma（ ICC） tissue.Methods： The expressions of GRP78 and IL-6 were detected by immunohistochemical technique in 48 cases of ICC,48 cases of hepatolithiasis and 30 cases of normal intrahepatic bile duct tissue. Results： The positive rates of GRP78 and IL-6 in ICC tissue were 64. 6% and 60. 4%,in hepatolithiasis tissue were 75. 0% and 68. 8%,respectively,which were significantly higher than those in normal intrahepatic bile duct tissue（ 6. 7% and 13. 3%,P〈0. 05）. The expression of GRP78 was positively correlated with that of IL-6（ rP= 0. 280,P = 0. 044） in ICC tissue. The expression of GRP78 was related with tumor histologic grade（ P = 0. 035）,lymph node metastasis（ P = 0. 015） and TNM stage（ P〈0. 01）. The patients with positive expression of GRP78 had shorter survival time than those with negative expression（ P〈0. 05）. The expression of IL-6 was associated with lymph node metastasis（ P = 0. 008） and TNM stage（ P = 0. 015）. There was no significant difference in survival curves of the patients with positive expression of IL-6 and those with negative expression（ P = 0. 331）. Conclusion： GRP78 and IL-6 may participate in the progress,growth and metastasis of ICC.

关 键 词：GRP78 IL-6 肝内胆管结石 肝内胆管癌 

分 类 号：R735.8[医药卫生—肿瘤]