CD4^＋CD25^＋T regs与CCL17和CCL22在头颈鳞状细胞癌发病机制中的初步研究  

The role of CD4^+CD25^+ T regs and CCL17,CCL22 in the pathogenesis of head and neck squamous cell carcinoma

作　　者：龚单春 张海东[2] 于振坤[2] 邢光前[1] GONG Shanchun ZHANG Haidong YU Zhenkun XING Guangqian(Department of Otolaryngology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China Department of Otolaryngology, Nanjing Tongren Hospital, Affiliated to School of Medicine, Southeast lInlvor~t,,~)

机构地区：[1]南京医科大学第一附属医院耳鼻咽喉科,南京210029 [2]东南大学医学院附属南京同仁医院耳鼻咽喉科,南京211100

出　　处：《临床耳鼻咽喉头颈外科杂志》2017年第20期1557-1560,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：南京市卫生局资助项目(No:YKK12216);南京市江宁区科技局资助项目(No:2012Ea17-2)

摘　　要：目的:探讨CD4^+CD25^+T regs与CCL17、CCL22在头颈部鳞状细胞癌(HNSCC)发病分子机制中的关系。方法:选取HNSCC手术患者20例,均为初发或治疗后(放化疗、手术)复发的患者。取术后大体标本的部分肿瘤原发灶作为试验组,同时取距肿瘤原发灶1~3cm的部分癌旁正常组织作为对照组。利用免疫荧光检测CD4^+/Foxp3及CD25^+/Foxp3,利用ELISA检测CCL17、CCL22。比较两组之间CD4^+、CD25^+以及CCL17、CCL22的差别,分析CD4^+、CD25^+与CCL17、CCL22之间的相关性。结果:试验组与对照组之间CD4^+/Foxp3、CD25^+/Foxp3平均光密度值差异有统计学意义(均P<0.05)。试验组与对照组之间CCL17、CCL22检测浓度值差异有统计学意义(均P<0.01)。试验组中CD25^+与CCL17、CCL22之间具有明显正相关(r=0.595、0.720,P<0.01)。结论:CD4^+CD25^+T regs与CCL17、CCL22在HNSCC发病的免疫机制中具有重要作用,两者相互影响,共同促进了HNSCC的复发和转移。Objective.. To investigate the role of CD4^＋CD25^＋ T regs and CCL17 and CCL22 in the pathogene- sis of HNSCC. Method：Twenty cases of HNSCC were enrolled. All patients were primary or recurrent after treat- ment （chemotherapy, surgery）. The primary tumor was taken as the experimental group, and the adjacent normal tissues from the primary tumor 1-3 cm were taken as control group. CD4^＋/Foxp3 and CD25^＋/Foxp3 were de- tected by immunofluorescence, while CCL17 and CCL22 were detected by ELISA. The difference and correlation between the amount of CD4^＋, CD25^＋and the expression of CCL17, CCL22 were observed and analyzed. Result~ The difference of mean optical density between CD4^＋/Foxp3 and CD25^＋/Foxp3 was statistically significant be- tween the experimental group and the control group （P〈0.05）. The concentration of CCL17 and CCL22 was sta- tistically different between the two groups （P〈0.01）. There was a positive correlation between CD25^＋ and CCL17, CCL22 （r=0. 595, 0. 720, P〈0.01）. Conclusion .CD4^＋CD25^＋ T regs and CCL17, CCL22 played an important role in the pathogenesis of head and neck squamous cell carcinoma, both of which interacted with each other, and promoted the recurrence and melaslasis of HNSCC.

关 键 词：头颈部肿瘤 癌 鳞状细胞 CD4^+CD25^+调节性T细胞 趋化因子CCLl7 趋化因子CCL22 

分 类 号：R739.91[医药卫生—肿瘤]