甲状腺乳头状癌与喉鳞状细胞癌代谢组学初步研究  

Preliminary findings for metabolite profiles of papillary thyroid carcinoma and laryngeal squamous cell carcinoma

作　　者：费梦嘉[1] 徐雅男[1] 王家东[1] FEI Mengjia XUYanan WANG Jiadong(Department of Head and Neck Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200001, China)

机构地区：[1]上海交通大学医学院附属仁济医院头颈外科,上海200001

出　　处：《临床耳鼻咽喉头颈外科杂志》2017年第20期1561-1565,共5页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：上海高校选拔培养优秀青年教师科研专项基金(No:101005.001.14.115)

摘　　要：目的:阐明甲状腺乳头状癌(PTC)与喉鳞状细胞癌(LSCC)的代谢组学特征并探讨其异同点。方法:联合应用气相色谱-飞行时间质谱和超高效液相色谱-飞行时间质谱技术,对57例PTC和33例LSCC患者的新鲜肿瘤组织及其邻近非肿瘤组织的代谢物进行检测,获取代谢谱。应用单维、多维统计学方法分析肿瘤组织与邻近非肿瘤组织之间的差异代谢产物以及相关的代谢通路。结果:PTC以及LSCC患者肿瘤组织与邻近非肿瘤组织分别存在46及41种差异代谢产物。两组患者共同的代谢特征主要表现在肿瘤组织中糖酵解、氨基酸代谢、一碳代谢及色氨酸代谢均较邻近非肿瘤组织明显增强。PTC与LSCC肿瘤组织中的嘌呤和嘧啶代谢产物较邻近非肿瘤组织增多,牛磺酸和次牛磺酸的代谢产物在PTC肿瘤组织中增多。脂肪酸代谢产物在PTC以及LSCC中又呈现共性的降低。结论:PTC与LSCC既具有肿瘤组织中糖酵解、氨基酸代谢、一碳代谢及色氨酸代谢明显增强的共性代谢特点,又具有各自独特的代谢特征,这些特征的揭示有助于阐明上述肿瘤的生物学特征。Objective：To identify distinct metabolite profiles of papillary thyroid cancer （PTC） and laryngeal squamous cell carcinoma （LSCC）. Method：Tumor and adjacent non-tumor specimens were collected from 57 PTC and 33 LSCC patients. Distinct metabolite profiles of tissues were examined using a combination of gas chromatog- raphy-time-of-flight mass spectrometry and ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry. Tb.e data were analyzed with multivariate statistical aaalysis to compare tb.e distinct metaholite profiles and related pathways of these three tumors. Result.. A panel of 46 and 41 differentially expressed metabo- lites were identified in tumor and adjacent non-tumor tissues of PTC and LSCC, respectively. Increased glycolysis, amino acids metabolism, one carbon metabolism and tryptophan metabolism were found in these two types of tumor tissues compared to adjacent non tumor tissues. Purine and pyrimidine metabolism was significantly elevated in PTC and LSCC tumor tissues, while taurine and hypotaurine were only higher in PTC tumor tissues. The fatty acid metabolism was detected at lower level in both PTC and LSCC tumor tissue. Conclusion：PTC and LSCC tumor tissues not only have common metabolic signatures characterized by increased glycolysis, amino acids metabolism, one carbon metabolism and tryptophan metabolism, but also have distinct metabolic signatures. It is helpful to un- derstand the nature of these two tumors.

关 键 词：代谢组学 甲状腺肿瘤 喉鳞状细胞癌 气相色谱-飞行时间质谱 超高效液相色谱-飞行时间质谱 

分 类 号：R736.1[医药卫生—肿瘤]