上颌窦黑色素瘤原位裸鼠模型的建立及其生物学特征  

Establishment and characterization of an orthotopic murine model of mucosal malignant melanoma of the maxillary sinus

作　　者：鞠思敏 陈飞 刘均 覃纲 谢亚柳[3] 王震玲 魏雪梅 JU Simin CHEN Fei LIU Jun QIN Gang XIE Yaliu WANG Zhenling WEI Xuemei(Department of Otolaryngology Head and Neck Surgery, the Affiliated Hospital of Southwest Medical University, Luzhou, 646000, China Department of Otolaryngology Head and Neck Surgery, West China Hospital Department of Otolaryngology Head and Neck Surgery, Chengdu No. 7 Peoplers Hospital State Key Laboratory of Biotherapy, West China Hospital Southwest Medi- cal University)

机构地区：[1]西南医科大学附属医院耳鼻咽喉头颈外科,四川泸州646000 [2]华西医院耳鼻咽喉头颈外科 [3]成都市第七人民医院耳鼻咽喉头颈外科 [4]华西医院生物治疗国家重点实验室 [5]西南医科大学

出　　处：《临床耳鼻咽喉头颈外科杂志》2017年第20期1599-1602,1605,共5页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：四川省科技厅支撑项目(No:2012SZ0024)

摘　　要：目的:建立稳定的上颌窦黑色素瘤原位裸鼠模型,为研究鼻腔鼻窦恶性肿瘤的发病机制及治疗手段提供动物模型。方法:将B16(小鼠黑色素瘤细胞)细胞悬液注射到裸鼠右上颌窦,待裸鼠形成原位瘤后,监测肿瘤的生长发展和荷瘤鼠的生存情况,并利用MRI技术观察肿瘤局部浸润情况。结果:在裸鼠上颌窦接种B16细胞,上颌窦出现原位瘤,裸鼠生存率和原位瘤体积大小与接种肿瘤细胞数量有关。MRI显示原位瘤向鼻腔、眼眶、颅底浸润。结论:成功构建了上颌窦黑色素瘤原位裸鼠模型,为进一步研究鼻腔鼻窦恶性肿瘤的生物学特征及新的治疗策略提供了可行的实验工具。Objective：To establish a stable and reliable orthotopic murine model of mucosal malignant melano- ma of the maxillary sinus so as to provide animal models for further studying for pathogenesis and therapy of si- nonasal malignancy. Method：B16 were implanted in the right maxillary sinus of male nude mice. After tumors ap- peared in right maxillary sinus, tumor growth and survival rate were recorded. The degree of tumor infiltration was observed through the MRI. Result： Mice with B16 implanted in right maxillary sinus developed primary tumors. The survival and the tumor volume were related to the number of tumor cells implanted. We found that the tumor can invade into nasal cavity, orbital cavity and the basilar region using MRI. Conclusion：We successfully established a model for mucosal malignant melanoma of the maxillary sinus. This model offers an experimental tool for further research on biological characteristics of sinonasal malignancy and the development of new therapy.

关 键 词：上颌窦 黑色素瘤 原位瘤模型 裸鼠 磁共振成像 

分 类 号：R739.62[医药卫生—肿瘤]