LRG1在结直肠癌患者血清中的表达及临床意义  

Expression of LRG1 in serum of patients with colorectal cancer and its clinical significance

作　　者：陈周峰[1] 黄智铭[1] 金瑞放[1] 

机构地区：[1]温州医科大学附属第一医院消化内科,浙江温州325000

出　　处：《中国现代医生》2017年第26期1-3,6,169,共5页China Modern Doctor

基　　金：浙江省温州市公益性科技计划项目(Y20140538)

摘　　要：目的研究富含亮氨酸的α-2糖蛋白1(Leucine rich alpha-2 glycoprotein 1,LRG1)在结直肠癌患者血清的表达,探讨其临床意义。方法共纳入65例结直肠癌患者、55例结直肠良性肿瘤患者、50例健康志愿者;分别采用酶联免疫吸附法(Enzyme linked immunosorbent assay,ELISA法)检测血清LRG1水平,然后对三组患者血清中LRG1和CEA表达水平、结直肠癌血清LRG1与临床肿瘤分期的关系进行统计分析。结果与结直肠良性肿瘤组及健康查体组相比,结直肠癌组血清中LRG1和CEA水平明显偏高(P<0.01),结直肠良性肿瘤组和正常对照组比较差异无统计学意义(P>0.05);结直肠癌肿瘤分期与患者血清LRG1水平相关,Ⅲ、Ⅳ期恶性肿瘤患者血清LRG1水平明显高于Ⅰ、Ⅱ期恶性肿瘤患者,差异有统计学意义(P<0.01);联合检测与单独检测LRG1相比,差异有统计学意义(Z=2.763,P<0.01);与单独检测相比,联合检测准确率更高,差异有统计学意义(Z=3.034,P<0.01);LRG1与CEA差异有统计学意义(Z=0.752,P<0.05)。结论 LRG1可作为结直肠癌诊断潜在的生物学标记。Objective To study the expression of leucine-rich alpha-2 glycoprotein 1（LRG1） in serum of patients with colorectal cancer and to explore its clinical significance. Methods A total of 65 patients with colorectal cancer, 55 patients with colorectal benign tumors and 50 healthy volunteers were enrolled. Serum LRG1 levels were measured by enzyme-linked immunosorbent assay（ELISA）, and then the levels of LRG1 and CEA in serum were compared between the three groups. The relationship between serum LRG1 and clinical stage was analyzed statistically. Results Compared with those of colorectal benign tumor group and healthy examination group, the levels of LRG1 and CEA in colorectal cancer group were significantly higher（P〈0.01）, and there was no significant difference between colorectal benign tumor group and normal control group（P〉0.05）. The stage of colorectal cancer was correlated with the level of serum LRG1.The level of serum LRG1 in patients with stage Ⅲ and Ⅳ malignant tumor was significantly higher than that in patients with stage Ⅰ and Ⅱ malignant tumor, and the difference was statistically significant（P〈0.01）. There was significant difference between the combined detection and separate detection of LRG1（Z=2.763, P〈0.01）. Compared with that of separate detection, the accuracy of combined detection was higher, and the difference was statistically significant（Z=3.034, P〈0.01）. There was significant difference between LRG1 and CEA（Z=0.752, P〈0.05）. Conclusion LRG1 can be used as a potential biomarker for the diagnosis of colorectal cancer.

关 键 词：结直肠癌 富含亮氨酸α-2糖蛋白1 癌胚抗原 肿瘤标志物 

分 类 号：R735.34[医药卫生—肿瘤] R730.43[医药卫生—临床医学]