家驴与藏野驴SRY基因多态性研究  

Polymorphism of SRY Gene between Equus kiang and Equus asinus

作　　者：王西峰[1] 晁生玉[1] 乌兰巴特尔 王照忠[1] 王成林[1] 王启菊[1] 赵森 马志杰[2] 党瑞华[3] 雷初朝[3] 

机构地区：[1]青海省海西州动物疫病预防控制中心,青海德令哈817099 [2]青海省畜牧兽医科学院,青海西宁810016 [3]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《家畜生态学报》2017年第9期21-24,共4页Journal of Domestic Animal Ecology

摘　　要：研究旨在分析家驴与藏野驴SRY基因多态性,为藏野驴的种质资源保护提供理论依据。通过提取2头青海家驴和2头藏野驴的耳组织或毛发DNA,PCR扩增并测序得到1038bp的SRY基因序列,包含5′端调控区330bp和整个编码区708bp。序列比对分析表明,藏野驴与青海家驴SRY基因序列间存在4个核苷酸差异(-265G>C,-131T>C,493G>A,629 A>C),其中后2个核苷酸差异发生在编码区,导致相应的氨基酸发生变化(Thr165Ala和Thr210Lys)。构建的系统进化树结果显示,马属动物中家驴先与藏野驴并为一支,后与各种野生斑马相聚,最后与家马聚为一支,提示藏野驴与家驴亲缘关系较近。The objective of this study is to amplify SRY gene in Equus kiang and explore the paternal genetic relationship among the genus Equus to provide a theoretical foundation for conservation of germ- plasm resources in Equus kiang . The genomic DNA of ear tissue or hair of two Equus kiang and two Qing-hai Equus asinus was extracted, and then was used to amplify and sequence 1038 bp fragment of SRY gene, which consists of 5-noncoding region of 330 bp and the whole coding region of 708 bp. The result of sequence alignment showed that there were four differences of SRY gene between Qinghai Equus asinus mA Equus kiang （-265G〉 C/G, -131T 〉 C/T , 4933G〉 A , 629A〉 C）, the latter two of which lead to change of amino acid （Thr 165 Ala, Thr 210 Lys）. The result of phylogenetic tree showed that paternal genetic relationship in the genus Equus was that Equus asinus firstly converged with Equus kiang , secondly converged with Equus burchellii, Equus grevyi and Equus zebra, lastly converged with Equus caballus, indicating the close relationship between Equus kiang and Equus asinus .

关 键 词：家驴 藏野驴 SRY基因 多态性 

分 类 号：S811.5[农业科学—畜牧学]