香加皮杠柳苷联用TRAIL对胃癌SGC-7901和MGC-803细胞的作用及其机制  

Effect of combined treatment of cortex periplocae and TRAIL on gastric cancer SGC-7901 and MGC-803 cells and its mechanism

作　　者：孙佳玮 魏思思[1] 董佩[1] 李磊[1] 戴素丽 赵连梅[1] 单保恩[1] 

机构地区：[1]河北医科大学第四医院科研中心,河北石家庄050000 [2]河北医科大学第四医院呼吸科,河北石家庄050000

出　　处：《中国肿瘤生物治疗杂志》2017年第10期1076-1080,共5页Chinese Journal of Cancer Biotherapy

基　　金：国家科学自然基金项目资助(No.81673642)~~

摘　　要：目的:探讨香加皮杠柳苷(cortex periplocae,CPP)联用肿瘤坏死因子相关凋亡诱导配体(tumor necrosis factor related apoptosis inducing ligand,TRAIL)对胃癌细胞的作用及其机制。方法:人胃癌细胞系SGC-7901、MGC-803培养完成后,采用50、100、200 ng/ml的CPP和1μg/ml的TRAIL单用或联合处理。MTS法检测SGC-7901和MGC-803细胞的增殖情况,流式细胞术检测其凋亡率,Western boltting检测pro-BID、Mcl-1、cleaved caspase-3、DR4、DR5的表达水平。结果:SGC-7901和MGC-803细胞经CPP(50、100、200 ng/ml)和TRAIL(1μg/ml)联合处理24 h后,细胞增殖率明显低于空白对照组和对应的CPP各剂量单独处理组(P<0.05或P<0.01)。SGC-7901和MGC-803细胞凋亡率均明显高于空白对照组和对应的CPP各剂量单独处理组(P<0.05或P<0.01)。SGC-7901和MGC-803细胞中pro-BID、Mcl-1表达水平明显降低(均P<0.05),cleaved caspase-3表达水平明显升高(P<0.05),DR4和DR5受体的表达水平升高(均P<0.05)。结论:CPP协同TRAIL可明显诱导人胃癌SGC-7901和MGC-803细胞凋亡,增强人胃癌细胞对TRAIL的敏感性。Objective： To investigate the effect of combined treatment of cortex periplocin （CPP） and tumor necro- sis factor related apoptosis inducing ligand （TRAIL） on gastric cancer cells and to explore the mechanism. Meth- ods： After routine culture, the gastric cancer cell lines （SGC-7901 and MGC-803） were treated with CPP （at concen- trations of 50,100,200 ng/ml） or TRAIL （1 μg/ml） or in combination of these two. The cell proliferation was detect- ed by MTS, the apoptosis was detected by Flow cytometry, and the expression levels of pro-BID, Mcl- 1, cleaved caspase-3, DR4 and DR5 were detected by Western blotting. Results： Compared with the control group and each CPP single treatment group, MTS assay demonstrated that CPP （50, 100, 200 ng/ml） in combination with TRAIL （1 ~tg/ml） significantly inhibited the proliferation of gastric cancer SGC-7901 and MGC-803 cell lines （all P〈0.05 or P〈0.01）. Flow cytometry demonstrated that the apoptosis rate of gastric cancer cells in combined treatment group was significantly higher than that of control group or each CPP single treatment group （P〈0.05 or P〈0.01）. Western blotting demonstrated that combined treatment for 24 h significantly decreased the expression of pro-BID and Mcl-1 （P〈0.05）, but increased the expression levels of cleaved caspase-3, DR4 and DR5（P〈0.05）. Conclusion： CPP in combination with TRAIL could significantly induce the apoptosis of gastric cancer SGC-7901 and MGC-803 cell lines and increase the susceptibility of cancer cells to TRAIL.

关 键 词：香加皮杠柳苷 肿瘤坏死因子相关凋亡诱导配体 胃癌细胞 增殖 凋亡 

分 类 号：R735.2[医药卫生—肿瘤] R730.5[医药卫生—临床医学]