CBX2蛋白在结直肠癌中的表达水平及临床意义  

Expression of chromobox homolog 2 in colorectal cancer and its clinical significance

作　　者：祝梓原 徐远东[1] 黎淑玲[1] 吴小兵[1] 刘学娟[1] 徐学虎[1] 

机构地区：[1]广州医科大学附属第三医院胃肠外科,广东广州510150

出　　处：《肿瘤》2017年第10期1056-1062,共7页Tumor

基　　金：广东省科技厅产学研协同创新成果转化项目(编号:2016B090918130)~~

摘　　要：目的:探讨色素框同源物2(chromobox homolog 2,CBX2)在结直肠癌组织和细胞中的表达情况及临床意义。方法:采用实时荧光定量PCR和蛋白质印迹法检测结直肠癌细胞、正常结直肠上皮细胞、结直肠癌组织和癌旁组织中CBX2 mRNA和蛋白的表达水平;采用免疫组织化学法检测66例结直肠癌及其对应的癌旁正常组织中CBX2的表达情况。分析CBX2表达与结直肠癌患者临床病理特征及预后的关系。结果:结直肠癌细胞中CBX2 mRNA和蛋白的表达水平均高于正常结肠上皮细胞FHC(P值均<0.05)。结直肠癌组织中CBX2 mRNA和蛋白的表达水平均高于癌旁组织(P值均<0.05);CBX2在结直肠癌组织中的阳性表达率为53.0%(35/66),明显高于癌旁正常组织中的7.6%(5/66),差异有统计学意义(P<0.05)。CBX2表达与结直肠癌患者的TNM分期、淋巴结转移、远处转移及生存时间显著相关(P值均<0.05)。CBX2高表达的结直肠癌患者的总生存时间短于低表达患者(P=0.01)。CBX2表达和远处转移是结直肠癌患者预后的独立影响因素(P值均<0.05)。结论:结直肠癌组织和细胞中CBX2过表达,在一定程度上参与了结直肠癌的发生和发展。并且CBX2的表达是结直肠癌患者预后的独立影响因素。Objective： To investigate the expression of chromobox homolog 2 （CBX2） gene in colorectal cancer （CRC） tissues and cells, and to explore its clinical significance. Methods： The expressions of CBX2 mRNA and protein in CRC cells, normal colorectal epithelial cells, CRC tissues and adjacent tissues were detected by real-time fluorescent quantitative PCR and Western blotting, respectively. The expression of CBX2 in CRC tissues and their corresponding adjacent normal tissues from 66 patients was detected by immunohistochemistry. The correlations of CBX2 expression with the clinicopathological features and prognosis of patients with CRC were analyzed Results： The expressions of CBX2 mRNA and protein in CRC cells were higher than those in normal colonic epithelial FHC cells （all P 〈 0.05）. The expression levels of CBX2 mRNA and protein in CRC tissues were also significantly higher than those in para-cancerous tissues （all P 〈 0.05）. The positive rate of CBX2 expression in CRC tissues was significantly higher than that in adjacent normal tissues （53.0% vs 7.6%, P 〈 0.05）. The expression of CBX2 was associated with TNM stage, lymph node metastasis, distant metastasis, and the survival status （all P 〈 0.05）. The overall survival time of CRC patients with high expression of CBX2 was shorter than that of CRC patients with low expression of CBX2 （P = O.01）. Both CBX2 expression and distant metastasis were independent prognostic factors in patients with CRC （both P 〈 0.05）. Conclusion： CBX2 is overexpressed in CRC tissues and cells, and is partly involved in the occurrence and development of CRC. Moreover, the expression of CBX2 is an independent prognostic factor for the patients with CRC.

关 键 词：结直肠肿瘤 基因表达 预后 色素框同源物2 

分 类 号：R735.3[医药卫生—肿瘤]