PDLIM4基因对胶质瘤预后及胶质瘤细胞放射敏感性的影响  

Effect of PDLIM4 gene on the prognosis of glioma and its cell radiosensitivity

作　　者：罗铭[1] 蔡望青[1] 李凯舒 刘家豪[2] 薛卫平 

机构地区：[1]中山大学孙逸仙纪念医院肿瘤放疗科,广州510120 [2]中山大学孙逸仙纪念医院神经外科,广州510120 [3]广州医科大学附属第六医院神经外科,清远511500

出　　处：《中华放射医学与防护杂志》2017年第10期725-729,762,共6页Chinese Journal of Radiological Medicine and Protection

基　　金：广东省科技计划项目（2014A020212091）

摘　　要：目的 分析PDLIM4（PDZ and LIM domain 4）基因与胶质瘤预后及放射敏感性的影响.方法 应用生物信息学技术对GSE53733芯片进行分析得出差异性表达基因,并使用免疫印迹法（Western blot）检测PDLIM4蛋白表达,实时荧光定量PCR、siRNA、MTT、流式细胞法检测、X射线照射等方法研究PDLIM4基因对胶质瘤预后及胶质瘤细胞照射敏感的相关性.结果 生物信息学分析结果提示PDLIM4在芯片中表达差异最明显（logFC=1.055897,P〈0.05）.通过40例胶质瘤的qPCR结果证实PDLIM4在高级别与低级别胶质瘤中差异表达（t=4.44,P〈0.05）,并与患者生存时间具有相关性（χ^2=5.52,P〈0.05）,且PDLIM4基因与胶质瘤细胞的放射敏感性相关（t=35.99,P〈0.05）.结论 PDLIM4基因表达水平与胶质瘤恶性程度及预后相关,并参与了细胞的X射线抵抗.Objective To investigate the effect of PDLIM4 （PDZ and LIM domain 4） gene on the prognosis and radiosensitivity of glioma. Methods The differentially expressed genes were analyzed by bioinformatics technique using GSE53733 gene chip. The expression of PDLIM4 protein was detected by Western blot. The effects of PDLIM4 gene on glioma prognosis and glioma cell radiosensitivity were studied by using real-time fluorescence quantitative PCR, siRNA, MTT and cell flow cytometry assays. Results PDLIM4 gene had the most significant differential expression in the chip （logFC=1. 055897, P〈0. 05）. The PCR assay of 40 glioma cases in our hospital confirmed that expression of PDLIM4 showed obvious difference between high- and low-grade gliomas （t =4. 44, P 〈0. 05）, which was correlated with the survival of patients （χ^2 =5. 52, P〈0. 05）. Moreover, PDLIM4 gene was involved in radioresistance of glioma cells （t = 35.99, P 〈 0.05）. Conclusions PDLIM4 gene expression level correlates with malignant degree and prognosis of glioma and also contributes to cell radioresistance.

关 键 词：PDLIM4基因 胶质瘤 恶性程度 预后 放射抵抗 

分 类 号：R730.55[医药卫生—肿瘤] R739.41[医药卫生—临床医学]