食管癌放射抗拒关键基因及通路的生物信息学分析  

Bioinformatics analysis of radioresistance key gene and pathway in esophageal carcinoma

作　　者：郭亚[1] 马红兵[1] 王宝峰[1] 任宏涛[1] 

机构地区：[1]西安交通大学第二附属医院肿瘤科,710004

出　　处：《中华放射医学与防护杂志》2017年第10期730-735,共6页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的 寻找食管癌放射抗拒关键分子及通路,从分子水平揭示食管癌放射抗拒发生机制.方法 从基因芯片公共数据库GEO中下载食管癌放射抗拒相关芯片数据（GSE61772,GSE61620）;进一步运用GEO2R进行差异基因分析,利用DAVID、String等分析软件对差异基因进行生物信息学分析.RT-PCR技术检测差异基因在不同放射敏感性细胞中表达差异.结果 两组芯片数据中共筛选出49个差异基因,这些基因参与生物学过程主要集中在调节多细胞生物合成、离子转运、DNA合成、代谢、调节细胞增殖等.差异基因涉及的主要生物学通路是Wnt信号通路.12个差异基因间存在相互作用关系,关键节点为CHN2.CHN2基因在不同放射敏感性食管癌细胞中表达未见明显差异.结论 包括CHN2在内的49个差异基因可能与食管癌放射抗拒发生相关,Wnt信号通路在其中具有重要作用.Objective To explore key gene and pathway of radioresistance in esophageal carcinoma and reveal its molecular mechanism of radioresistance. Methods The gene expression profiles of GSE61772 and GSE61620 were downloaded from the Gene Expression Omnibus （GEO）. Differentially expressed genes （ DEGs ） were screened by GEO2R. Gene Ontology （ GO ） enrichment, Kyoto Encyclopedia of Genes and Genomes （ Kegg ） enrichment and protein-protein interaction （ PPI ） network construction were performed by DAVID and String softwares. RT-PCR was used to detect the differences in the expression of different genes in different radiosensitivity cells. Results A total of 49 differentially expression genes were screened. These genes were mainly involved in the regulation of multicellular biosynthesis, ion transport, DNA synthesis, metabolism, cell proliferation and so on. The major biological pathways included a Wnt signal pathway. 12 DEGs interacted with each other, and CHN2 may be a key node. The expression of CHN2 gene had no obvious difference between TE13R and TE13. Conclusions 49 differentially expressed genes, including CHN2, may be involved in radioresistance of esophageal carcinoma, and the Wnt signaling pathway may be an important pathway in this radioresistance.

关 键 词：食管癌 放射抗拒 基因芯片公共数据库 生物信息学 

分 类 号：R730.55[医药卫生—肿瘤] R735.1[医药卫生—临床医学]