蝙蝠葛碱在人乳腺癌MCF-7细胞对5氟尿嘧啶敏感性的研究  

Dauricine enhances the sensitivity of 5-fluorouracil in human breast cancer MCF-7 cells

作　　者：李红阳 孙亮[2] 姜醒[3] 苑光军[3] 刘艳[1] 

机构地区：[1]哈尔滨医科大学附属第四医院肿瘤内科,哈尔滨150001 [2]哈尔滨医科大学人体解剖学教研室 [3]黑龙江中医药大学佳木斯学院

出　　处：《实用肿瘤学杂志》2017年第5期385-389,共5页Practical Oncology Journal

基　　金：黑龙江省自然科学基金面上项目(H201464);黑龙江省高校专项基金(003000166)

摘　　要：目的探讨人乳腺癌MCF-7细胞受蝙蝠葛碱(Dauricine,Dau)作用后,对5氟尿嘧啶(5-FU)敏感性的影响。方法分别以终浓度为2.5μg/mL的蝙蝠葛碱、50μg/mL的5-FU、2.5μg/mL的蝙蝠葛碱和50μg/mL的5-FU作用于人乳腺癌细胞MCF-7,运用MTT法检测细胞增殖活性,Transwell实验分析细胞的迁移,DAPI染色检测细胞的凋亡,Western blot法检测cyclinD1和Bcl-2蛋白的表达。结果MTT实验结果显示联合使用阈下浓度的蝙蝠葛碱增强了5-FU对细胞增殖的抑制;Transwell实验表明联合应用阈下浓度的蝙蝠葛碱进一步加剧了5-FU对细胞迁移的抑制;DAPI染色说明联合应用阈下剂量的蝙蝠葛碱加强了5-FU对细胞凋亡的诱导,Western blot实验表明联合应用阈下浓度的蝙蝠葛碱进一步抑制了cyclinD1和Bcl-2蛋白的表达。结论蝙蝠葛碱可有效增强人乳腺癌MCF-7细胞对5-FU的敏感性。Objective The objective of this study was to investigate the effect of dauricine on the sensitivity of 5-fluorouracil（ 5-FU） in human breast cancer MCF-7 cells. Methods MCF-7 cells were treated with 2. 5 μg/mL of dauricine,50 μg/mL of 5-FU,or 2. 5 μg/mL of dauricine with 50 μg/mL of 5-FU,the cell proliferation was measured by MTT assay. The cell migration was determined by Transwell assay; The cell apoptosis was detected by DAPI staining; The expression of cyclinD1 and Bcl-2 gene was examined by Western blot.Results The results showed that the combination of subthreshold concentration of dauricine enhanced the inhibitory effect of 5-FU on proliferation in MCF-7 cells. The combined use of subcutaneous concentration of dauricine further aggravated the inhibitory effect of 5-FU on cell migration. The combination of subcapsular dauricine enhanced the induction of apoptosis by 5-FU. The combination of dauricine with 5-FU could inhibit the expression of cyclinD1 and Bcl-2 protein in MCF-7 cells. Conclusion Dauricine can effectively enhance the sensitivity of 5-FU in human breast cancer MCF-7 cells.

关 键 词：蝙蝠葛碱 5氟尿嘧啶 乳腺癌 MCF-7细胞 

分 类 号：R737.9[医药卫生—肿瘤]