s腺苷蛋氨基酸通过抑制P13K/AKT/mTOR通路调控HepG2肝癌细胞自噬作用研究  

The Effect of Regulating Autophagy of S-adenosyl Methionine on Hep G2 Hepatoma Cells by Inhibiting P13K/AKT/mTOR Pathway

作　　者：佟辉[1] 李涛[1] 申川[1] 彭承宏[1] 邱伟华[1] 沈柏用[1] 祝哲诚[1] TONG Hui LI Tao SHEN Chuan PENG Chenghong QIU Weihua SHEN Baiyong ZHU Zheeheng(Department of General Surgery, Ruo＇in Hospital Affiliated to School of Medicine of Shanghai Jiao Tong University, Shanghai 200025. Chin)

机构地区：[1]上海交通大学医学院附属瑞金医院普外科,上海200025

出　　处：《中国继续医学教育》2017年第23期205-207,共3页China Continuing Medical Education

摘　　要：目的探讨s腺苷蛋氨基酸通过抑制P13K/AKT/mTOR通路调控HepG2肝癌细胞自噬作用。方法选取上海生命科学研究院细胞资源中心的HepG2肝癌细胞作为研究标本,随机分为阴性对照组、阳性对照组和观察组,并比较三组间HepG2肝癌细胞的增殖、凋亡情况以及HepG2肝癌细胞自噬相关标记物记LC3B和Bclin1表达水平。结果 s腺苷蛋氨基酸作用24 h、48 h和72 h时抑制HepG2细胞增殖的IC_(50)分别为24.34μmol/L、10.26μmol/L、6.24μmol/L,与阴性对照组和阳性对照组比较,差异均具有统计学意义(P<0.05);10μmol/L、30μmol/L和100μmol/L不同浓度s腺苷蛋氨基酸作用于HepG2细胞48 h后出现的凋亡率分别为(26.28±1.34)%、(22.34±1.82)%和(11.18±0.60)%,而阴性对照组其HepG2细胞48 h后的凋亡率为(6.28±0.02)%,即观察组凋亡率随着药物浓度的增高而降低,且与阴性对照组比较,观察组各浓度s腺苷蛋氨基酸作用于HepG2细胞48h后出现的凋亡率均明显较高,即s腺苷蛋氨基酸具有诱导HepG2细胞凋亡的作用(P<0.05);经流式细胞仪结果显示,不同浓度s腺苷蛋氨基酸作用于HepG2细胞48 h后其LC3B和Bclin1水平均呈逐渐降低(P<0.05)。结论 s腺苷蛋氨基酸可通过抑制P13K/AKT/mTOR通路调控进一步增强HepG2肝癌细胞自噬作用。Objective To investigate the effect of regulating autophagy of S-adenosyl methionine on HepG2 hepatoma cells by inhibiting P13K/AKT/ mTOR pathway. Methods HepG2 cells were selected from Shanghai Life Science Research Institute cell resource center as the research samples and randomly divided into negative control group, positive control group and the observation group. The HepG2 liver cancer cell proliferation, apoptosis and HepG2 cell autophagy related markers LC3B and Bclinl expression were compared among 3 groups. Results The proliferation IC50 of HepG2 cells at 24 h, 48 h and 72 h by S-adenosine methionine inhibition was 24.34 μmol/L, 10.26 μmol/L and 6.24 μmol/L respectively, which were significantly different with that in the negative control group and positive control group （P〈0.05）. The apoptosis of HepG2 cells at 10 μmol/L, 30 μmol/L and 100 μmol/L concentrations of S-adenosyl methionine after 48h were （26.28±1.34）%, （22.34±1.82）% and （11.18±0.60）%, and the apoptotic rate of HepG2 cells in the negative control group was （6.28 ± 0.02）% after 48 h, the apoptotic rate of the observation group decreased with the increase of the drug concentration. Compared with the negative control group, the apoptotic rate of HepG2 cells was significantly higher than that of HepG2 cells （P 〈 0.05）. The result of flow cytometry showed that the LC3B and Bclinl levels were gradually decreased with increased concentrations of S-adenosyl methionine in Hep （32 cells after 48 h （P〈0.05）. Conclusion The S-adenosyl methionine can improve the autophagy of HepG2 hepatoma cells by inhibiting P 13K/AKT/mTOR pathway.

关 键 词：s腺苷蛋氨基酸 P13K/AKT/mTOR HEPG2肝癌细胞 自噬作用 

分 类 号：R735[医药卫生—肿瘤]