二氢杨梅素对裸鼠腹腔移植人胃癌细胞中Caveolin-1表达的影响  

Effect of Dihydromyricetin on caveolin-1 in nude mouse models of human gastric cancer

作　　者：王佑权 张帆 周寿红[2] 

机构地区：[1]南华大学附属第二医院肿瘤外科,湖南衡阳421001 [2]南华大学医学院生理学教研室,湖南衡阳421001

出　　处：《中国现代医学杂志》2017年第24期11-16,共6页China Journal of Modern Medicine

基　　金：湖南省教育厅科研项目(No:11c1108)

摘　　要：目的观察二氢杨梅素(DMY)对裸鼠腹腔移植人胃癌细胞生长的抑制作用及对腹腔瘤组织中Caveolin-1表达的影响。方法复制人胃癌腹腔移植的裸鼠模型。将复模成功的24只裸鼠随机分为4组,即移植瘤模型组,DMY低、中及高剂量处理组。分别观察荷瘤裸鼠的腹腔移植瘤的生长和成瘤情况,测量肿瘤的重量、长短径并计算肿瘤的体积。采用免疫组织化学法和Western blot检测人胃癌腹腔移植瘤腹腔瘤体中Caveolin-1和Ki67的表达。结果与移植瘤模型组比较,100和200 mg/(kg·d)DMY中、高剂量组腹腔移植瘤成瘤个数,腹腔瘤体的重量及腹腔瘤体的体积均降低,呈现剂量依赖性(P<0.05),裸鼠的食欲、活动情况和精神状态等一般情况改善。与移植瘤模型组比较,100和200 mg/(kg·d)DMY中、高剂量组腹腔移植瘤体表达Caveolin-1的阳性细胞百分率和Caveolin-1的蛋白表达水平均增加,呈现剂量依赖性(P<0.05);而Ki67的阳性细胞百分率和Caveolin-1的蛋白表达水平均降低,也呈现剂量依赖性(P<0.05)。结论二氢杨梅素以剂量依赖的方式抑制人胃癌腹腔移植瘤的生长,其作用机制可能与上调Caveolin-1的表达有关。Objective To investigate inhibitory effect of Dihydromyricetin （DMY） on the growth of gastriccancer and expression of caveolin-1 in nude mouse models of human gastric cancer. Methods Nude mousemodels of peritoneal transplanted human gastric cancer was established. Twenty-four successfully transplantednude mice were randomly divided into 4 groups： tumor group, tumor with low dose group [50 mg/ （kg· d） ],moderate dose group [100 mg/ （kg· d） ] and high dose group [200 mg/ （kg·d） ] of DMY. The growth rate andtumor size were monitored. The expression levels of caveolin -1 and Ki67 in tumors were tested byImmunohistochemistry and Western blot. Results The number, weight, and volume of tumors were significantlydecreased in both tumor with moderate and high dose groups dose dependently when compared with thetumor group（ 〈 0.05）. The appetite, activity and mental state of animals in the tumor with moderate and highdose groups were significantly improved in comparison with the tumor group. Immunohistochemistry andWestern blot data indicated that the percentage of Caveolin-1 positive cells and expression level of proteinCaveolin-1 were significantly increased in the tumor with moderate and high dose groups when compared withthe tumor group dose dependently （ 〈 0.05）. Similarly, the percentage of Ki67 positive cells and expressionlevel were significantly increased in the moderate and high dose groups when compared with the tumor groupdose dependently （ 〈 0.05）. Conclusions DMY inhibits the growth of peritoneal transplanted human gastriccancer in dose dependent manner and the mechanism may be related to upregulation of Caveolin-1.

关 键 词：二氢杨梅素 胃癌 裸鼠 腹腔移植瘤 小凹蛋白-1 

分 类 号：R735.2[医药卫生—肿瘤]