食管癌患者miR-182表达水平及其对预后的影响  

Expression of miRNA-182 and its effect on prognosis of esophageal carcinoma

作　　者：鲁之中[1] 李军民[1] 胡云芝[2] 李晓辉[3] 李卫[1] 姜富国 李帅 申辰 

机构地区：[1]河南省焦作市人民医院检验科,河南焦作454002 [2]河南省焦作市人民医院儿科,河南焦作454002 [3]河南省焦作市人民医院肿瘤外科,河南焦作454002

出　　处：《中国现代医学杂志》2017年第24期39-43,共5页China Journal of Modern Medicine

摘　　要：目的探讨miR-182在食管癌患者组织中的基因表达及其对预后的影响。方法采用实时荧光定量PCR检测59例食管癌标本和癌旁组织中miR-182表达水平;并分析其表达水平与患者的临床病理特征和随访结局之间的关系。结果相较于癌旁组织,食管癌组织中miR-182表达水平增高(P<0.05);miR-182表达水平与肿瘤分化程度、浸润深度、是否发生转移以及临床分期相关(P<0.05),与性别、年龄以及肿瘤大小无关系(P>0.05);生存分析显示miR-182高表达组总体生存期低于低表达组(P<0.05);Cox多因素回归分析结果显示miR-182表达水平可作为食管癌患者预后不良的标志之一。结论 miR-182在食管癌组织中高表达,其高表达提示预后较差,可作为食管癌潜在的预测分子标志物。Objective To investigate the concentration of miRNA-182 in esophageal carcinoma and itsassociation with disease prognosis. Methods MicroRNA-182 in 59 esophageal carcinoma tissues and relatedadjacent healthy tissues were measured by quantitative real time polymerase chain reaction （qRT -PCT）.Association of miRNA -182 with clinical characters including pathologic types, and clinical outcome wasdetermined. Results The concentration of miRNA -182 in esophageal carcinoma tissues was significantlyincreased when compared with that in adjacent healthy tissues （ 〈 0.05）. Overexpression of miRNA -182correlated closely with tumor differentiation level, depth of infiltration, metastasis, and clinical stage （ 〈 0.05）.Kaplan -Meier survival analysis indicated that patients with high miRNA -182 experienced worse outcomecompared with others （ 〈 0.05）. Moreover, multivariate analysis showed that increase of miRNA-182 was anindependent predictor of overall survival（ 〈 0.05）. Conclusions MicroRNA-182 is overexpressed in esophagealcarcinoma tissue and concentration of miRNA-182 is closely correlated with poor outcome, suggesting thatmiRNA-182 may serve asa prognostic biomarker for esophageal carcinoma.

关 键 词：miR-182 食管癌 预后 实时荧光定量PCR 

分 类 号：R735.1[医药卫生—肿瘤]