姜黄素对人乳腺癌SKBR3细胞株的抑制作用及机制研究  

Inhibitory effect of curcumin on human breast cancer cell line SKBR3 and its mechanism

作　　者：应佳可 叶宇[1] 方迪龙[1] 

机构地区：[1]杭州市红十字会医院普外科,浙江杭州310003

出　　处：《中国生化药物杂志》2017年第10期15-17,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的观察中药姜黄素对乳腺癌SKBR3细胞株增殖、凋亡、Caspase-3活性及端粒酶的影响,探讨姜黄素的作用机制。方法采用MTT法绘制细胞生长曲线;PI荧光和Annexin V荧光双染检测凋亡细胞;采用Westernblot法检测Caspase-3的表达;采用TRAP-PCR银染法检测端粒酶活性。结果姜黄素对SKBR3细胞增殖具有明显的抑制作用,呈时间和浓度依赖性,对SKBR3细胞端粒酶活性具有一定的降低作用,可提高Caspase-3表达水平,诱导细胞凋亡。结论姜黄素能够有效抑制SKBR3细胞增殖,增加Caspase-3蛋白的合成,抑制端粒酶的活性而介导SKBR3细胞凋亡。Objective To observe the effect of curcumin on proliferation,apoptosis,Caspase-3 activity and telomerase activity of chronic granulocytic breast cancer SKBR3 cell line,and to investigate the mechanism of curcumin.Methods The cell growth curve was drawn by MTT;the apoptotic cells were detected by Annexin,V and PI double staining;the expression of Caspase-3 was detected by Westernblot;the activity of telomerase was detected by TRAP-PCR silver staining.Results Curcumin could significantly inhibit the proliferation of SKBR3 cells in a time and concentration dependent,has a certain effect on reducing the telomerase activity of SKBR3 cells can enhance the expression of Caspase-3 induced apoptosis.Conclusion Curcumin can effectively inhibit the proliferation of SKBR3 cells,increase the synthesis of Caspase-3 protein,inhibit the activity of telomerase and mediate the apoptosis of SKBR3 cells.

关 键 词：姜黄素 SKBR3细胞 端粒酶 CASPASE-3 凋亡 

分 类 号：R361.3[医药卫生—病理学]