碳纳米微粒免疫标记层析技术在粪便隐血、钙卫蛋白、癌胚抗原联检中的应用设计  

The application design of carbon nanoparticle immunomarker chromatography in fecal occult blood,calcium and cancer embryo antigen

作　　者：王永兵[1] 程斌[2] 徐斌[1] 李刚[1] 凌杰[1] 张文忠[1] 

机构地区：[1]上海市浦东新区人民医院肛肠外科,上海200120 [2]上海市浦东新区人民医院检验科,上海200120

出　　处：《中国生化药物杂志》2017年第10期26-28,31,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：上海市浦东新区科技创新基金（PKJ2014-Y30）; 上海市医学重点专科（2015-B-18）

摘　　要：目的设计一种基于纳米碳微粒免疫层析技术的粪便隐血、钙卫蛋白、癌胚抗原快速联检的方法。方法应用在微量毒品检测中研制的纳米碳侧向流免疫层析技术,选择人血红蛋白、钙卫蛋白、癌胚抗原合适的双抗体,将胶体碳探针标记的三个检测物的1抗固化在碳垫上,与相应检测物配对的另一株2抗固定在硝酸纤维素膜的检测区域（T线）,质控线（C线）由羊抗鼠IgG抗体组成,粘合吸水垫、硝酸纤维膜、胶体碳释放垫和样品垫,烘干切割制成检测卡。选择100例肠镜检查阴性者和100例结直肠癌患者肠道准备前粪便标本,0.9%氯化钠溶解离心上清液滴入试纸条装入专用卡槽,应用自主研发的即时检定量检测仪分别检测粪便隐血、钙卫蛋白、癌胚抗原含量。结果研发的粪便隐血、钙卫蛋白、癌胚抗原纳米碳侧向流免疫层析检测卡应用方便,即时检定量检测仪可读数据区间达到0～7 000,灵敏度可达到0.2 ng/mL,肠镜检查阴性者和结直肠癌患者粪便隐血阳性率分别为1.91%和77.01%,钙卫蛋白平均含量分别为（26.81±5.96）μg/g和（589.00±42.29）μg/g、癌胚抗原平均含量分别为（1.21±0.45）μg/g和（234.32±11.24）μg/g。结论纳米碳微粒免疫层析技术的快速联检方法可靠,粪便隐血、钙卫蛋白、癌胚抗原联检对结直肠癌筛查有应用价值。Objective To design a method for rapid detection of fecal occult blood,calcium and cancer embryo antigen based on the immunochromatography of nanoparticles.Methods Used nano carbon lateral flow immune chromatography technology which applicated in trace detection of drug development,choose human hemoglobin,calcium,protein,carcinoembryonic antigen suitable double antibody.1 resistance of three test taged by colloidal carbon probe cured in carbon mat,and the corresponding test pairs of another plant 2 fixed on nitrocellulose membrane resistance test region（Line T）,quality control line（Line C） which composed of sheep anti-mouse IgG antibody,glue water cushion,nitrocellulose membrane,colloidal carbon release pad and sample pad,drying cutting made test card.Choose 100 cases of endoscopic check negatie and 100 cases of colorectal cancer patients with intestinal preparation before stool specimens,dropped the dissolved the centrifugal supernatant in normal saline into the strip and put the special card slot,applied independent research of real-time quantitative detector checked the content of fecal occult blood,the calcium content of protein,carcinoembryonic antigen respectively.Results The application of nano carbon lateral flow immune chromatography test card for fecal occult blood calcium,protein,carcinoembryonic antigen was conveniented.Real-time quantitative detector check readable data range of 0-7000,the sensitivity data of 0.2 ng/mL.The positive rate of endoscopic check negatie and colorectal cancer patients with fecal occult blood were 1.91% and 77.01% respectively,the average content of calprotectin were（26.81±5.96） μg/g and（589.00±42.29） μg/g.Average content of carcinoembryonic antigen were（1.21±0.45） μg/g and（234.32±11.24） μg/g respectively.Conclusion The rapid detection method of nanometer carbon particle immunochromatography was reliable,and fecal occult blood,calcium protamine and cancer embryo antigen jiont test was value for colorectal cancer screening.

关 键 词：纳米碳 免疫层析 粪便 结直肠癌 

分 类 号：R735.37[医药卫生—肿瘤]