胃癌腹膜转移的血清蛋白质特异性生物标志物的研究  被引量:3

Study on serum protein specific biomarkers in gastric carcinoma patients with peritoneum metastasis

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作  者:纪托 陈海莲[2] 周中银[3] JI Tuo CHEN Hai-lian ZHOU Zhong-yin(Huangshi Renfu Hospital Hubei, Huangshi 435004, China Huangshi Manicipal Center for Disease Con trol and Prevention, Huangshi 435000, China Hubei Provincial People' s Hospital, Wuhan 430060, China)

机构地区:[1]黄石人福医院,湖北黄石435004 [2]黄石市疾病预防控制中心,湖北黄石435000 [3]湖北省人民医院,湖北武汉430060

出  处:《中国中西医结合消化杂志》2017年第9期702-704,708,共4页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

摘  要:[目的]筛选胃癌腹膜转移中可能存在的特异性蛋白标记物。[方法]采用CM10蛋白质芯片和表面增强激光解析电离飞行时间质谱(SELDI-TOF-MS)技术,对19例胃炎患者和18例不同期别胃癌患者的的血清蛋白质谱进行分析。[结果]胃癌患者与胃炎患者相比,7种蛋白质明显过表达(P<0.05,Student t检验)。其中,发现3268Da,3397Da,3927Da和4129Da的四个蛋白质仅在患有腹膜转移的患者中差异表达。采用CART(分类和回归树)方法成功地将胃癌腹膜转移患者与无腹膜转移的患者分离。[结论]这4个蛋白峰有可能与胃癌腹膜转移相关,或将成为其诊断的特异性生物蛋白标志物。[Objective]To screen specific protein markers that might exist in peritoneal carcinogenesis of gastric cancer.[Methods]The serum protein spectra of 19 patients with gastritis and 18 patients with different stages of gastric cancer were analyzed by CM10 protein chip and surface enhanced laser desorption timeof-flight mass spectrometry(SELDI-TOF-MS).[Results]Seven proteins were significantly overexpressed in patients with gastric cancer compared with gastritis patients(P〈0.05,Student t test).Among them,the four proteins that 3268 Da,3397Da,3927 Da and 4129 Da were differentially expressed only in patients with peritoneal diffusion.CART(classification and regression tree)method was used to successfully isolate gastric cancer patients with peritoneal transmission from patients without peritoneal sowing.[Conclusion]These four protein peaks may be associated with peritoneal transmission of gastric cancer,or will be the specific biological protein markers for their diagnosis.

关 键 词:SELDI-TOF-MS技术 胃癌 腹膜转移 

分 类 号:R735.2[医药卫生—肿瘤]

 

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