Micro PET/CT显像评价裸鼠MDA-MB231乳腺癌乏氧与增殖状态的研究  

Research on micro PET/CT imaging in evaluating tumor hypoxic and proliferation in MDA-MB231 breast cancer in nude mice

作　　者：余文静 徐慧琴[1] 谯凤 苏晓雨 张丹[1] 薛杨央[1] 

机构地区：[1]安徽医科大学第一附属医院核医学科,合肥230022

出　　处：《安徽医学》2017年第10期1236-1239,共4页Anhui Medical Journal

基　　金：国家自然科学基金项目(基金项目:81371587)

摘　　要：目的探讨18F-FMISO、18F-FDG micro PET/CT显像评价裸鼠MDA-MB231乳腺癌移植瘤中肿瘤乏氧与增殖状态的可行性。方法 16只裸鼠MDA-MB231乳腺癌移植瘤模型先后进行18F-FDG及18F-FMISO PET/CT显像并采集肿瘤最大标准化摄取值[SUVmax(FDG)、SUVmax(FMISO)],所有模型显像结束后立即处死,取肿瘤组织行HIF-1α及Ki67免疫组织化学染色,将SUVmax值与HIF-1α、Ki67的表达水平进行相关性分析。结果 18F-FMISO平均SUVmax值1.69,18F-FDG平均SUVmax值5.80。SUVmax(FMISO)与HIF-1α、Ki67的表达均呈正相关性(r=0.74、0.52,P<0.05)。SUVmax(FDG)与Ki67的表达呈正相关性(r=0.86,P<0.05),与HIF-1α表达无相关性。HIF-1α与Ki67蛋白的表达呈正相关性(r=0.60,P<0.05)。结论 18F-FMISO PET/CT显像可同时对活体肿瘤进行乏氧及增殖的可视化监测,18F-FDG可提供有效的肿瘤增殖显像,但不能作为有效的肿瘤乏氧显像剂。Objective To investigate the feasibility ofl8F - FMIS0 and 18F - FDG Micro PET/CT imaging in evaluating tumor hy-poxic and cell proliferation inMDA - MB231 breast cancer in nude mice. Methods Sixteen nude mice bearing human breast cancer （MDA231 cell line） xenografttumors were monitored with 18F -FDG PET/CT and 18F - FMIS0 PET/CT imaging. The SUVma（FMIS0） and SUVma（FDG） were collected respectively. Mice were randomly sacrificed after PET/CT imaging,then immunohistochemical was used to assess HIF - la and Ki67 expression. We investigated the correlations of Ki67expression. Results The averageof SUVma（ FMIS0） was 1.69,while the averageof SUVmax（FDG） was 5. 80. Both the expression of HIF - la and Ki67 wererelated with SUVmax（FMISO） （r =0.74, P〈0.05, r=0.52, P〈0.05, respectively）. SUVmax（ FDG） was signifi-cantlycorelated with Ki67（ r = 0. 86, P 〈0. 05） . No correlation was detected between SUVmax（ FDG） a d HIF - laexpression. The expression of HIF - 1α was also correlated with K67 （ r = 0.60, P 〈 0. 05 ） Conclusion The uptake of FMISOisclosely correlated with the expressions of Ki67 and HIF - 1α,18F - FMISO PET/CT plays an important role in visual detection of tumor hypoxic and cell proliferation. The uptake of FDG is-significantlycorrelated with Ki67,which ca reflect tumor proliferation effectively, but not as an effectively hypoxie imaging agent.

关 键 词：脱氧葡萄糖 细胞增殖核抗原Ki67 乏氧诱导因子-1Α 动物模型 乳腺癌 

分 类 号：R730.44[医药卫生—肿瘤] R737.9[医药卫生—临床医学]