miR-210调控Mex3B蛋白对肺癌细胞侵袭和增殖的影响  

Effects of miR-210 on Mex3B protein on migration and proliferation of lung cancer cells

作　　者：王俊钢[1] 李聪聪[2] 毛广显 张洁[1] 杨翠[1] 

机构地区：[1]郑州大学附属医院/南阳市中心医院胸外科,南阳473000 [2]郑州大学附属医院/南阳市中心医院特需病房一病区,南阳473000 [3]北京大学附属深圳医院胸外科,深圳518036

出　　处：《中国免疫学杂志》2017年第10期1468-1473,共6页Chinese Journal of Immunology

基　　金：深圳市科技计划项目(JCYJ20140415162338820);深圳市医疗卫生科研项目(201302068)

摘　　要：目的:探讨miR-210和Mex3B蛋白对肺癌细胞侵袭和增殖能力的影响。方法:运用q PCR检测miR-210在正常肺组织和癌旁组织中的表达情况;运用q PCR检测Mex3B在肺癌组织、癌旁组织中的表达;q PCR检测miR-210在不同肺癌细胞株中(A549、H1299、H1650和H358)的表达水平;双荧光素酶报告基因系统检测miR-210对Mex3B转录的影响;细胞活性实验检测miR-210的表达对肺癌细胞活性的影响;平板克隆实验检测miR-210的表达对肺癌细胞A549的增殖能力的影响;Transwell侵袭实验检测miR-210的表达对肺癌细胞株A549的侵袭能力的影响。结果:和癌旁组织比较,miR-210在肺癌组织中表达明显增高,和癌旁组织比较,Mex3B在肺癌中表达较低,双荧光素酶报告基因系统检测结果显示,miR-210可以直接调控Mex3B的转录活性,抑制miR-210的表达活性后,A549肺癌细胞的细胞活性受到一定的抑制;抑制miR-210后,肺癌细胞株A549的侵袭和增殖能力明显降低。结论:miR-210可以靶向调控肺癌细胞的侵袭和增殖能力。Objective： To investigate the effect of miR-210 on the migration and proliferation of lung cancer cells. Methods：The expression of miR-210 in normal lung tissues and lung cancer tissues were detected by q PCR. The expression of Mex3 B in lung cancer tissues and adjacent tissues were detected by q PCR. q PCR was used to detect the expression of miR-210 in different lung cancer cell lines（ A549,H1299,H1650 and H358）. The effect of miR-210 on the transcription of Mex3 B was detected by double luciferase reporter gene system. The effect of miR-210 expression on the activity of lung cancer cells was detected by cell viability assay. The effect of miR-210 on the proliferation of A549 cells were detected by plate cloning assay. Transwell invasion assay were used to detect the effect of miR-210 on the invasion ability of lung cancer cell line A549. Results： Compared with adjacent tissues,the expression of miR-210 were significantly decreased in lung cancer tissues,Mex3 B were lower in lung cancer. Double luciferase reporter gene system results showed that miR-210 can directly regulate the transcriptional activity of Mex3 B. The invasion and proliferation ability of lung cancer cell line were significantly reduced after inhibition of miR-210. Conclusion： miR-210 can target regulate the expression of Mex3 B,and affects the invasion and proliferation of lung cancer cells.

关 键 词：肺癌 MIR-210 Mex3B TRANSWELL 

分 类 号：R734.2[医药卫生—肿瘤]