基于UHPLC/Q-TOF/MS分析羊耳菊有效组分的入血成分  

Analysis of components in plasma from effective fractions of Inula cappa based on UHPLC/Q-TOF/MS

作　　者：巩仔鹏[1,2,3,4] 陈亭亭 侯靖宇 吴林霖[1,2,3,4] 李梅 李月婷 陈思颖[1,2,3,4] 兰燕宇 

机构地区：[1]贵州医科大学贵州省药物制剂重点实验室,贵州贵阳550004 [2]贵州医科大学民族药与中药开发应用教育部工程研究中心,贵州贵阳550004 [3]贵州医科大药学院心,贵州贵阳550004 [4]贵州医科大学国家苗药工程研究中心,贵州贵阳550004

出　　处：《中国药理学通报》2017年第11期1605-1610,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81360680);贵州省优青项目[黔科合人字(2015)11号];贵州省教育厅“民族药与中药开发应用产学研基地建设”项目(黔科合KY字[2013]122);贵州省科学技术基金项目[黔科合LH字(2015)7362];贵州省研究生卓越人才计划项目(黔教研合ZYRC字[2014]012);贵州省中医药管理局项目(No QZZY-2015-078)

摘　　要：目的应用UHPLC/Q-TOF/MS技术和血清药物化学研究手段,分析检测羊耳菊有效组分的入血成分。方法大鼠灌胃给予羊耳菊有效组分提取物后,腹主动脉抗凝采血,血浆经甲醇二次沉淀蛋白处理。采用Eclipse Plus C18RRHD色谱柱(2.1 mm×100 mm,1.8μm),0.1%甲酸水(A)-0.1%甲酸乙腈(B)做流动相梯度洗脱,流速0.3 mL·min-1。负离子检测模式,同时结合布鲁克道尔顿公司的Metabolite Tools软件,鉴定和表征其血中的移行成分。结果给药后,在大鼠血浆中共检测到16个代谢产物,包括9个原型成分和7个代谢物,代谢方式主要以咖啡酰基奎宁酸类化合物的异构化、甲基化及甲基葡萄糖醛酸化的复合反应为主。结论本研究较为全面地阐释了羊耳菊活性部位提取物在大鼠血中的入血成分,为进一步研究羊耳菊药材的体内过程和药效物质基础提供了一定的参考。Aim To analyze the main components of effective fractions of Inula cappa in rat plasma with UHPLC/Q-TOF/MS technology and serum pharmacochemistry theory. Methods After gavage administration with Inula cappa extracts, blood was collected from abdominal aorta,and the protein in plasma sample was precipitated by methanol. Eclipse Plus C18 RRHD( 2. 1 mm × 100 mm,1. 8 μm) was used,with 0. 1% formic acid agueous solution( A)-0. 1%formic acid and acetonitrile( B) as the mobile phase for gradient elution,and the flow rate was 0. 3 m L ·min-1. Detected at negative ion mode,and with the help of Metabolite Tools software from Bruker Corporation,components in plasma were defined. Results A total of 16 compounds were identified,including 9 prototypes and 7 metabolites. Main metabolites were isomerization,methylation,glucuronidation and recombination of caffeoylquinic acid. Conclusions Therefore,our study has comprehensively expounded Inula cappa extracts' constituents migrating to rat plasma,and provided a reference for further studies for in vivo metabolic process and effective material base of Inula cappa.

关 键 词：羊耳菊 血浆 入血成分 UHPLC/Q-TOF/MS 血清药物化学 代谢物 

分 类 号：R-332[医药卫生] R282.71