超声造影对兔眼脉络膜黑色素瘤血流循环的定量评估  

Quantitative evaluation of microcirculation in choroidal melanoma of rabbits using contrast-enhanced ultrasound

作　　者：高敏[1] 唐金华 刘海芸[1] GAO Min TANG Jin-Hua LIU Hai-Yun

机构地区：[1]上海交通大学附属第一人民医院眼科,上海市200080 [2]上海交通大学附属第一人民医院眼科超声科,上海市200080

出　　处：《眼科新进展》2017年第11期1005-1009,共5页Recent Advances in Ophthalmology

基　　金：上海市科委一般项目基金资助(编号:15411952700)~~

摘　　要：目的探索改良兔眼脉络膜黑色素瘤原位肿瘤模型的建立方法及成瘤影响因素;观察超声造影在定性和定量评估脉络膜黑色素瘤血液循环模式中的应用。方法将脉络膜黑色素瘤的瘤组织碎片植入10只兔的右眼,左眼作为对照。4周后采集兔眼普通超声、多普勒彩色超声、超声造影图像并自动记录,使用Sono Liver分析眼内肿块血流充盈曲线。结果兔眼内脉络膜黑色素瘤成瘤率为80.0%(2只动物死亡)。肿块体积最大切面两条垂直线测量值为(0.92±0.28)cm、(0.66±0.30)cm,肿瘤的峰值强度为(263.56±143.33)%,上升时间为(5.01±1.42)s,达峰时间为(6.17±2.18)s,渡越时间为(18.01±7.65)s,曲线下面积为(4505.13±1491.75)%,上升支斜率为59.48±75.18,下降支斜率为-7.21±5.76。肿瘤组织与正常眼球壁组织比较,峰值强度、曲线下面积、下降支斜率均较大(均为P<0.05),上升时间、上升支斜率、达峰时间、平均渡越时间差异均无统计学意义(均为P>0.05)。结论脉络膜下腔植入肿瘤组织碎片是一种稳定兔眼脉络膜黑色素瘤模型建立方法,超声造影在定性和定量判断眼脉络膜黑色素瘤内血流微循环模式中具有重要的临床价值。Objective To explore a modified method for establishing a rabbit model of B16F10 cells and the factors in tumorigenesis as well as to observe contrast-enhanced ultrasonography （CEUS） in qualitative and quantitative evaluation of dynamic circulation pattern of choroidal melanoma.Methods Tumor fragments of choroidal melanoma were implanted into the suprachoroidal space of the right eyes in 10 rabbits,with the left eyes as the controls.After 4 weeks,rabbits eyes were harvested for examined using ultrasonic imaging,color doppler flow imaging,and CEUS,and the images were analyzed with SonoLiver software.Results The proportion of rabbits with choroidal melanoma formation was 80.0%,with 2 cases dead.The maximum major diameter of the tumor was （0.92±0.28）cm and （0.66±0.30）cm.The maximum intensity,rise time,time to peak,mean transit time and area under curve was （263.56±143.33）%,（5.01±1.42）s,（6.17±2.18）s,（18.01±7.56）s,（4505.13±1491.75）% respectively,while the rising slope and descending slope was 59.48±75.18 and -7.21±5.76 accordingly.And the peak intensity,area under curve and descending slope of choroidal tumor was larger than those of normal eyes （all P〈0.05）,while no significant difference was found in rise time,time to peak,mean transit time and rising slope （all P〉0.05）.Conclusion Suprachoroidal implantation of tumor fragments can facilitate the formation of the stable animal model of choroidal melanoma.And CEUS application has a great clinical value in qualitative and quantitative evaluation of blood circulation in melanoma.

关 键 词：脉络膜黑色素瘤 动物模型 超声造影 血流循环 

分 类 号：R773.4[医药卫生—眼科]