姜黄素对人脉络膜黑色素瘤细胞株血管形成拟态抑制作用及其相关机制的研究  

Inhibitory effects of curcumin on vasculogenic mimicry in choroid melanoma cell line OCM-1 and its mechanisms

作　　者：高文[1] 陈陆霞[2] 何彦津 梁凤鸣[1] 

机构地区：[1]天津中医药大学第一附属医院眼科,天津市300193 [2]天津市眼科医院,天津市300000 [3]天津医科大学眼科医院,天津市300000

出　　处：《眼科新进展》2017年第11期1018-1021,共4页Recent Advances in Ophthalmology

摘　　要：目的研究姜黄素对人脉络膜黑色素瘤细胞株OCM-1血管形成拟态(vasculogenic mimicry,VM)及其PI3K信号通路的影响。方法三维培养人脉络膜黑色素瘤细胞株OCM-1,观察其形成VM的能力。观察梯度浓度(0μmol·L^(-1)、10μmol·L^(-1)、20μmol·L^(-1)、30μmol·L^(-1)、40μmol·L^(-1))的姜黄素对OCM-1细胞株VM形成的影响,采用免疫细胞化学检测参与VM形成的PI3K与EPHA2的表达。结果姜黄素在体外可以抑制OCM-1细胞株VM的形成,呈浓度依赖性。姜黄素对OCM-1细胞Eph A2和PI3K蛋白表达的影响:Eph A2和PI3K蛋白表达随姜黄素(10μmol·L^(-1)、20μmol·L^(-1)、30μmol·L^(-1)、40μmol·L^(-1))浓度的升高呈浓度依赖性降低。各组分别与0μmol·L^(-1)姜黄素组比较,差异均有统计学意义(均为P=0.000),其中30μmol·L^(-1)姜黄素组和40μmol·L^(-1)姜黄素组比较,差异无统计学意义(P=0.300、P=0.188)。结论姜黄素能明显抑制OCM-1细胞株VM的形成,其机制可能与下调EPHA2、PI3K蛋白的表达,从而抑制PI3K信号通路有关。Objective T o observe the effects of curcumin( Cur) on vasculogenic mimicry( VM) and PI3 K pathway in choroid melanoma cell line OCM-1 in vitro 3-dimentional culture.Methods T hree-dimensional culture of O C M-1 cells was performed for the observation of V Mformation and the influence of concentration gradient( 0 μmol·L^(-1),10 μmol·L^(-1),20μmol·L^(-1),30 μmol·L^(-1),40 μmol·L^(-1)) Cur on VM,and immunohis to chemistry staining was used to detect the expression of PI3 K and Eph A2 in O C M-1 cell line.Results C ur could markedly inhibit the formation of V Min choroid melanoma cell line O C M-1 in vitro 3-dimensional culture with a dose-dependent manner.Cur suppressed EphA2 and PI3K expression in a concentration-dependent manner,and the difference between groups with 0 μmol·L^(-1) Cur group was significant statistically( all P = 0. 000),but there was no significant difference between 30 μmol·L^(-1) and 40 μmol·L^(-1) group( Eph A2,P = 0.300; PI3 K,P = 0.188). Conclusion Cur can significantly inhibit V Mformation in choroid melanoma cell line O C M-1,of which the mechanismmay be related to the downregulation expression of Eph A2 and PI3 K,thus inhibiting PI3 K signaling pathway.

关 键 词：脉络膜黑色素瘤 姜黄素 血管形成拟态 PI3K 

分 类 号：R773[医药卫生—眼科]