帕金森病模型小鼠脾悬液中辅助性T细胞17和髓系抑制性细胞数量的变化及其意义  

Changes and their significances of the numbers of T helper 17 cells and Myeloid-derived suppressor cells in the spleen suspension of model mouse with Parkinson's disease

作　　者：陈思远[1] 舒扬[2] 赵峰[1] 于明[1] CHEN Si-yuan SHU Yang ZHAO Feng et al(Department of Neurology, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China)

机构地区：[1]江苏大学附属医院神经内科,镇江在读硕士研究生212001 [2]江苏大学附属医院中心实验室,镇江212001

出　　处：《临床神经病学杂志》2017年第5期369-372,共4页Journal of Clinical Neurology

基　　金：江苏省自然科学基金(BK20160548);江苏省"333工程"科研项目(BRA2014173);江苏省"六大人才高峰"科研项目(WSN038);镇江市重点研发计划(SH2016032);镇江市社会发展科技计划项目(SH2015047)

摘　　要：目的探讨帕金森病(PD)模型小鼠脾悬液中辅助性T细胞17(Th17)和髓系抑制性细胞(MDSC)数量的变化及其意义。方法将40只C57BL/6小鼠随机分为PD组(A组)、PD+吉西他滨组(B组)、吉西他滨组(C组)与对照组(D组),每组10只小鼠。A组和B组予1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)(30 mg/kg)连续腹腔注射7 d;B组和C组于第1 d,7 d腹腔注射吉西他滨(120 mg/kg);D组予等体积生理盐水腹腔注射。第8 d进行行为学实验后,检测各组小鼠黑质区酪氨酸羟化酶(TH)阳性细胞数量和脾悬液Th17和MDSC细胞百分率。结果与建模前比较,A组、B组小鼠调头时间、爬杆时间明显延长,悬挂时间明显缩短(均P<0.05)。与C组、D组比较,建模后A组、B组小鼠调头时间、爬杆时间明显延长,悬挂时间明显缩短(均P<0.05)。A组、B组小鼠中脑黑质区TH阳性细胞数量较C组和D组明显减少(均P<0.05)。A组、B组小鼠脾悬液中Th17、MDSC细胞百分数较C组和D组明显升高(均P<0.05);且B组小鼠脾悬液中Th17细胞百分数明显高于A组(P<0.05),A组小鼠脾悬液中MDSC细胞百分数明显高于B组(P<0.05)。Pearson相关性分析结果显示,A组和B组小鼠Th17和MDSC细胞百分数间均无相关性(r=-0.319,r=0.04,均P>0.05)。结论 PD模型小鼠Th17、MDSC数量增加,可能参与了PD的发生发展。MDSC有可能发挥了抑制Th17扩增的免疫调节作用。Objective To observe the changes and their significances of the numbers of T helper 17 cells（ Th17） and Myeloid-derived suppressor cells（ MDSC） in the spleen suspension of model mouse with Parkinson＇s disease（ PD）. Methods Forty C57 BL/6 mouse were randomly divided into PD group（ A group）,PD ＋Gemcitabine group（ B group）,Gemcitabine group（ C group） and control group（ D group）,with 10 mouse in each group. Mouse in group A and B were injected intraperitoneally with 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine（ MPTP）（ 30 mg/kg） for 7 d continuously. Mouse in group B and C were injected intraperitoneally with Gemcitabine（ 120 mg/kg） at 1 d and 7 d. After behavioral experiment at 8 d,the number of tyrosine hydroxylase（ TH） positive cells in the substantia nigra and the cell percentage of Th17 and MDSC in the spleen suspension of mouse in each group were detected. Results Compared with before modeling,the turning around time and pole climbing time of mouse in A group and B group after modeling were significantly longer,while the suspension time was significantly shortened（ all P〈0. 05）. Compared with C group and D group,the turning around time and pole climbing time of mouse in A group and B group after modeling were significantly longer,while the suspension time was significantly shortened（ all P〈0. 05）. Compared with C group and D group,the numbers of TH positive cells in substantia nigra of mouse in A group and B group were significantly reduced（ all P〈0. 05）. Compared with C group and D group,the cell percentages of Th17 and MDSC in the spleen suspension of mouse in A group and B group were significantly increased（ all P〈0. 05）. The cell percentage of Th17 in the spleen suspension of mouse in B group was significantly higher than A group（ P〈0. 05）,while the cell percentage of MDSC in the spleen suspension of mouse in A group was significantly higher than B group（ P〈0. 0 5）. Pearson correlation analysis results showed that there was n

关 键 词：帕金森病 辅助性T细胞17 髓系抑制性细胞 1-甲基-4-苯基-1 2 3 6-四氢吡啶 吉西他滨 

分 类 号：R-332[医药卫生] R742.5