双氢青蒿素对人下咽癌裸鼠移植瘤的抑制作用  

Antitumor Effect of Dihydroartemisinin on Hypopharyngeal Cancer in Nude Mice with Transplanted Tumor

作　　者：石新丽 李圣豪[2] 白静 王泽明 张天民 李路亚 李光星 李晓明 SHI Xinli LI Shenghao BAI Jing WANG Guangxing LI Xiaoming ZHANG Tianmin LI Luya LI Zeming(Department of Otolaryngology Head and Neck Surgery, Bethune International Peace Hospital, Shijiazhuang 050082, China Department of Pathobiology and Immunology, Hebei University of Chinese Medicine. Shiiiazhu,Tnc, 0~0091 t~l,,,,~)

机构地区：[1]白求恩国际和平医院耳鼻喉头颈外科,石家庄050082 [2]河北中医学院免疫学与病原生物学教研室,石家庄050021

出　　处：《肿瘤防治研究》2017年第10期647-651,共5页Cancer Research on Prevention and Treatment

基　　金：2016年度中国博士后科学基金面上项目(2016M603018);河北省自然科学基金(H2017423011);河北省大学生创新创业训练计划(201614432031)

摘　　要：目的明确不同浓度双氢青蒿素(DHA)抑制人下咽癌细胞Fadu裸鼠移植瘤生长的效应。方法细胞克隆形成实验分析DHA体外抑制肿瘤形成能力;建立Fadu裸鼠移植瘤模型,腹腔注射低、中、高浓度DHA作为DHA处理组,腹腔注射顺铂作为化疗组;测量移植瘤的体积,干预3周后剥离瘤块,判断不同浓度DHA抑瘤率及抑瘤效果;根据体重变化及肝、脾、肾的重量变化,判断DHA是否有潜在的毒性。结果 (1)DHA抑制Fadu细胞的体外生长;(2)DHA低(25 mg/kg)、中(50mg/kg)、高(100 mg/kg)剂量组的抑瘤率分别为39.74%、60.52%、78.70%,顺铂组的抑瘤率为79.22%;(3)DHA对裸鼠体重及肾重量无影响,能增加荷瘤裸鼠的肝、脾重量。结论低、中剂量DHA虽能抑制Fadu移植瘤生长,但抑瘤效果不如顺铂;高剂量DHA抑瘤效果与顺铂相同,且没有明显的肝、肾、脾毒性作用。Objective To investigate the effect of different concentrations of dihydroartemisinin （DHA） on human hypopharyngeal cancer Fadu cells in nude mice. Methods Clonogenic assay was applied to determine whether DHA （10, 20 and 40μmol/L） for 24h affected long-term colony formation. Nude mice with xenograft tumor model bearing Fadu cells were intraperitoneally injected into low, medium and high concentrations of DHA as DHA groups, while intraperitoneal injection of cisplatin as the chemotherapy group. The volume of transplanted tumor was measured. After three weeks, the xenograft tumors were removed and weighted, then, the inhibitory rates were determined. The potential toxicity was determined through the weight changes of mice, liver, spleen and kidney. Results （1） DHA inhibited the growth of Fadu cells in vitro. （2） The inhibitory rates of low- （25mg/kg）, medium- （50mg/kg） and high- （100mg/kg） dose DHA groups were 39.74 %, 60.52 % and 78.70%, while, 79.22% in cisplatin group. （3） DHA had no effect on body and kidney weight of nude mice. DHA increased the weight of liver and spleen in tumor-bearing nude mice. Conclusion Low- and medium-dose DHA noticeably inhibit the growth of Fadu xenograft tumor in vivo, but the effect is not as good as cisplatin. High dose of DHA has the same anti-tumor effect as cisplatin, without observable toxic effect on liver, kidney or spleen.

关 键 词：双氢青蒿素 下咽癌 Fadu细胞 裸鼠移植瘤 

分 类 号：R739.63[医药卫生—肿瘤]