Protective effects of Taxifolin on H_2O_2-induced damage in H9C2 cells  

作　　者：HUANG Jun-gang ZHAO Ming-yi WANG Xiao-li SU Qi-li CAI Qian LI Peng ZHU Ping 黄俊刚;赵明一;王晓莉;苏其利;蔡骞;李鹏;朱平(Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong General Hospital, Guangdong Academy of Medical Sciences;Department of Pediatrics, The Third Xiangya Hospital of Central South University)

机构地区：[1]Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong General Hospital, Guang- dong Academy of Medical Sciences, Guangzhou, 510100, China [2]Department of Pediatrics, The Third Xiangya Hospital of Central South University, Changsha 410013, China.

出　　处：《South China Journal of Cardiology》2017年第3期209-214,共6页岭南心血管病杂志（英文版）

基　　金：supported by National Natural Sciences Foundation of China(No.81370230 and81570279);Natural Sciences Foundation of Hunan Province(No.2015JJ6118);Grant from the New Xiangya Talent Project of the Third Xiangya Hospital of Central South University(No.JJ201524);Medical Scientific Research Foundation of Guangdong Province(No.A2016392);Industry-University-Research Cooperation Innovation Major Project of Guangdong Province(No.1561000143)

摘　　要：Background Taxifolin（Tax） is an essential natural antioxidant. Multiple studies have shown that Tax can protect cardiomyocytes from ischemia-reperfusion injury. However, the underlying mechanism is still unclear.Methods H9C2 cells were randomly divided into control, H_2O_2 group, Tax pretreatment group（Tax ＋ H_2O_2）;Tax effect group. Cell activity was detected by CCK-8 and the intracellular structure was observed by transmission electron microscopy. Autophagy was determine by Western blotting analysis of Beclin-1, Bcl-2 and PKC.Results Tax pretreatment significantly increased anti-apoptotic protein Bcl-2 and autophagy protein Beclin-1.Expression of PKC was inhibited by Tax. Conclusions Tax pretreatment could protect H9 C2 cells against H_2O_2-induced damage through the Bcl-2 and autophagy pathways.Background Taxifolin（Tax） is an essential natural antioxidant. Multiple studies have shown that Tax can protect cardiomyocytes from ischemia-reperfusion injury. However, the underlying mechanism is still unclear.Methods H9C2 cells were randomly divided into control, H_2O_2 group, Tax pretreatment group（Tax ＋ H_2O_2）;Tax effect group. Cell activity was detected by CCK-8 and the intracellular structure was observed by transmission electron microscopy. Autophagy was determine by Western blotting analysis of Beclin-1, Bcl-2 and PKC.Results Tax pretreatment significantly increased anti-apoptotic protein Bcl-2 and autophagy protein Beclin-1.Expression of PKC was inhibited by Tax. Conclusions Tax pretreatment could protect H9 C2 cells against H_2O_2-induced damage through the Bcl-2 and autophagy pathways.

关 键 词：TAXIFOLIN AUTOPHAGY PKC pathway anti-oxidative effect. 

分 类 号：R541[医药卫生—心血管疾病]