贵阳地区育龄人群HbA_2筛查轻型β-地中海贫血截断值的确定  

Determination of Cut-off Values of HbA_2 Screening for Mild β-thalassemia in Guiyang Population of Reproductive Age

作　　者：韩文平 王树辉[3] 徐琴[1] 杨楠楠 韩媛媛 黄凌 黄盛文[3] 安邦权[3] HAN Wenping WANG Shuhui XU Qin YANG Nannan HAN Yuanyuan HUANG Ling HUANG Shengwen AN Bangquan(School of Clinical Laboratory Science, Guizhou Medical University, Guiyang 550004, Guizhou , China Department of Clinical Laboratory, People~ Hospital of Guizhou Province, Guiyang 550002, Guizhou, China)

机构地区：[1]贵州医科大学医学检验学院,贵州贵阳550004 [2]贵州医科大学 [3]贵州省人民医院检验科,贵州贵阳550002

出　　处：《贵州医科大学学报》2017年第10期1121-1124,1129,共5页Journal of Guizhou Medical University

基　　金：国家自然科学基金(81660023);贵州省科学技术厅基础计划[黔科合人才团队(2015)4019号];贵阳市科技创新平台计划[筑科合同(20161001)35]

摘　　要：目的:建立贵阳地区育龄人群血红蛋白A_2(HbA_2)筛查轻型β-地中海贫血的截断值。方法:对152例育龄期男性,129例育龄期女性非孕妇和110例孕妇进行血红蛋白电泳和β-地中海贫血基因检测;以基因检测为金标准,绘制Hb A_2的ROC曲线,确定各组HbA_2筛查轻型β-地中海贫血的截断值。结果:整体的截断值为3.95%,敏感度、特异度和准确度分别为94.8%、99.3%和98.5%;男性组的HbA_2截断值(4.40%)明显高于女性非妊娠组(3.75%)和女性妊娠组(3.70%),女性妊娠状态对HbA_2截断值无明显影响。结论:建立了贵阳地区育龄人群HbA_2筛查轻型β-地贫的截断值,不同性别育龄人群的Hb A_2截断值存在差异。Objective： To determine the cut-off values of hemoglobin A2（ HbA2） screening for mildβ-thalassemia in Guiyang population of reproductive age. Methods： Hemoglobin electrophoresis andβ-thalassemia gene tests were carried out in 152 men of reproductive age,129 non-pregnant women of reproductive age,and 110 pregnant women. With genetic testing as the gold standard,receiver operating characteristic（ ROC） curves of HbA2 were drawnto determine the cut-off values of HbA2 screening for mild β-thalassemia in each group. Results： The overall cut-off valueof HbA2 was 3. 95%,and sensitivity,specificity,accuracy were 94. 8%,99. 3%,98. 5% respectively. The cut-off value of HbA2 in male group（ 4. 40%） was significantly higher than those of female non-pregnancy group（ 3. 75%）and female pregnancy group（ 3. 70%）. Women in pregnancy status had no obvious influence on cutoff value of HbA2. Conclusion： The cut-off values of HbA2 screening for mild β-thalassemia in reproductive population in Guiyang region have been determined. There is certain difference of HbA2 cut-off value in terms of gender,which has a certain guiding significance inscreening for mild β-thalassemia.

关 键 词：Β-地中海贫血 血红蛋白A2 ROC曲线 截断值 性别 贵阳 

分 类 号：R556.7[医药卫生—血液循环系统疾病]