结直肠癌中LAMP2a编码蛋白的表达及临床病理意义  

Expression and Clinicopathological Significance of LAMP2a in Colorectal Cancer

作　　者：时帅 窦建华[2] 唐光波[2] 赵爽 聂勇战[2] 郑华川 

机构地区：[1]锦州医科大学附属第一医院肿瘤基础与转化实验室,辽宁锦州121000 [2]第四军医大学西京消化病医院消化内科,西安710032

出　　处：《中国医科大学学报》2017年第11期970-975,共6页Journal of China Medical University

基　　金：国家自然科学基金(81472544;81672700);辽宁省科技攻关项目(2015408001);辽宁省百千万人才项目(2015408001)

摘　　要：目的阐明结直肠癌发生发展过程中溶酶体相关膜蛋白2a(LAMP2a)的表达及临床病理意义。方法利用免疫组化检测组织芯片上结直肠癌、正常黏膜和腺瘤中LAMP2a的表达水平,探讨结肠直肠癌临床病理特征与生存时间的关系。结果结直肠癌和腺瘤中LAMP2a表达高于正常黏膜(P<0.05),LAMP2a表达与结直肠癌TNM分期、淋巴结转移呈负相关(P<0.05),与淋巴管浸润、肝转移、浸润深度和分化程度不相关(P>0.05)。Kaplan-Meier分析显示LAMP2a表达与结直肠癌预后良好不相关(P>0.05)。单因素生存分析显示,淋巴管浸润、血管浸润、淋巴结转移、TNM分期、肝转移、远处转移和分化等与结直肠癌患者预后差相关(P<0.05)。Cox比例危险回归模型分析表明,浸润深度和远处转移是影响结直肠癌患者生存时间的重要因素(P<0.05)。结论 LAMP2a表达参与结直肠癌发生和演进过程,可能作为结直肠癌恶性生物学行为的分子标志物。Objective To investigate the clinicopathological and prognostic significance of lysosome-assaciated membrane protein-2a （LAMP2a） expression in the development of colorectal cancer. Methods Immunohistochemistry was performed to detect the LAMP2a expression in colorectal cancer,normal mucosa,and adenoma tissues. Results LAMP2a expression in colorectal cancer and adenoma was higher than that in normal mucosa （P 〈 0.05）. Further, LAMP2a expression in eoloreetal cancer was negatively correlated with TNM staging and lymph node metastasis （P 〈 0.05） ;however,the invasion and liver metastasis, depth of invasion and differentiation had no correlation with the lymph node （P 〉 0.05）. Kaplan-Meier analysis showed that LAMP2a expression in coloreetal cancer is not significantly correlated with good prognosis （P 〉 0.05）. Univariate survival analysis showed that lymphatic invasion, vascular invasion, lymph node me- tastasis, TNM stage, liver metastasis, distant metastasis, and differentiation were correlated with poor prognosis of colorcctal cancer （P 〈 0.05）. The Cox proportional hazards regression model showed that the depth of invasion and distant metastasis were important factors affecting the survival period of patients with colorectal cancer （P 〈 0.05）. Conclusion LAMP2a may play a role in the carcinogenesis and progression of eolorectal cancer, and may be used as a molecular marker for the biological behavior of eolorectal cancer.

关 键 词：结直肠癌 溶酶体相关膜蛋白2a 肿瘤发生 演进 预后 

分 类 号：R735.3[医药卫生—肿瘤]