北方汉族缺血性脑卒中人群ABCB1基因rs1045642多态性与氯吡格雷抵抗的相关性研究  

The Correlation between the ABCB1 rs1045642 Polymorphism and Clopidogrel Resistance among Chinese Han Patients with Ischemic Stroke

作　　者：胡畔[1] 王加璐[1] 何志义[1] 

机构地区：[1]中国医科大学附属第一医院神经内科,沈阳110001

出　　处：《中国医科大学学报》2017年第11期1019-1023,共5页Journal of China Medical University

摘　　要：目的探讨中国北方汉族缺血性脑卒中人群ABCB1基因rs1045642多态性与氯吡格雷抵抗(CR)之间可能存在的关系。方法选取110例急性缺血性脑卒中患者,入院后口服氯吡格雷(75 mg/d),于首日给药前及服用药物7 d时分别采集静脉血(2m L),测定血小板聚集率。CR定义为2次血小板聚集率的变化值不超过10%。所有患者根据结果被分为CR组和非CR(NCR)组。提取基因组DNA后用PCR扩增目的基因并用RFLP法测定SNP。结果 ABCB1基因rs1045642位点TT基因型频率CR组与NCR组比较明显升高(P=0.003)。相比于携带C等位基因,携带T等位基因频率在CR组明显高于NCR组(P=0.001)。结论北方汉族缺血性脑卒中患者中,ABCB1基因在SNP位点rs1045642的多态性可能是CR发生的一个独立危险因素,T等位基因可能是CR发生的风险基因。Objective To investigate the relationship between the ABCB1 rs1045642 polymorphism and clopidogrel resistance （CR） among Chinese Han patients with isehemie stroke. Methods In total, 110 patients with acute ischemic stroke were included in the study. Venous blood samples （2 mL） were collected after oral administration of 75 mg/d clopidogrel;the platelet aggregation rate was measured before elopidogrel administration and after 7 days of elopidogrel administration. CR is defined as no more than 10% change in the platelet aggregation rate over two times . Based on the results,patients were divided into the CR group and non-CR （NCR） group. Genomie DNA was extracted,the target gene was amplified by PCR,and the SNP was determined by RFLP. Results The TT genotype frequency of ABCB1 rs1045642 was significantly higher in the CR group than in the NCR group （P = 0.003）. T allele carriers in CR group show much more （57.1% vs 34%,P = 0.001） compared with those in the NCR group. Conclusion The ABCB1 rs1045642 polymorphism may be an independent risk factor and the T allele may be a gene indicating risk for CR among Chinese Han patients with ischemic stroke.

关 键 词：缺血性脑卒中 ABCB1基因 单核苷酸多态性 rs1045642 氯吡格雷抵抗 

分 类 号：R743.3[医药卫生—神经病学与精神病学]