基于木瓜蛋白酶过氧化物模拟酶活性的尿酸检测  

Application of papain as peroxidase mimetics to uric acid detection

作　　者：万冬梅 郑鹄志[1] 隆异娟[1] WAN Dong-mei ZHENG Hu-zhi LONG Yi-juan(School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715)

机构地区：[1]西南大学化学化工学院,重庆400715

出　　处：《分析试验室》2017年第10期1132-1135,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(21405124);西南大学中央高校基本业务费(XDJK2012C048)资助

摘　　要：木瓜蛋白酶具有过氧化物模拟酶活性,可以催化过氧化氢氧化3,3',5,5'-四甲基联苯胺(TMB)产生蓝色的显色反应。结合尿酸酶和木瓜蛋白酶构建了一个比色检测尿酸(UA)的新方法。其检测过程受pH、温度、TMB浓度和反应时间的影响,最优的检测条件分别为pH 3.0、温度为30℃、TMB浓度0.80 mmol/L及反应时间2 h,线性范围为20~200μmol/L,检出限2.4μmol/L。实验证实了该方法具有较好的选择性并且已经成功地应用于人血清样品的尿酸测定。Papain possessed peroxidase-like activity, which could catalyze the oxidation of the peroxidase substrate 3,3＇,5,5＇-tetramethylbenzidine （TMB） by H2O2 to a blue colored product. A new eolorimetric method was developed for uric acid （UA） detection using urease and papain. The detection is influenced by pH, temperature, concentration of TMB, reaction time, and the optimal condition were 3.0, 30℃ , 0. 80 mmol/L and 2 h respectively. As low as 2.4 μmol/L UA could be detected with a linear range from 20 μmol/L to 200 μ moL/L. It proved that this method has good selectivity and the proposed method was successfully applied to the determination of UA in human serum samples.

关 键 词：木瓜蛋白酶 尿酸 比色检测 

分 类 号：Q503[生物学—生物化学]