GnIH对去卵巢兼予雌激素大鼠下丘脑POMC的调节作用  

REGULATING EFFECTS OF GNIH ON POMC IN HYPOTHALAMUS OF OEP RATS

作　　者：魏萌[1] 司丽娜[1] 陈磊[1] 杨松鹤[1] 程露阳[1] 乔跃兵[1,2] 

机构地区：[1]承德医学院,河北承德067000 [2]沧州医学高等专科学校

出　　处：《承德医学院学报》2017年第6期458-460,共3页Journal of Chengde Medical University

基　　金：河北省自然科学基金资助项目(H2013406115);河北省科技厅计划项目(08276101D-20);河北省高等学校科学研究项目(QN2015121);河北省卫生厅医学科学研究项目(20130012)

摘　　要：目的:探讨侧脑室微量注射促性腺激素抑制激素(GnIH)对下丘脑阿片-促黑素细胞皮质素原(POMC)蛋白表达的影响。方法:40只雌性SD大鼠建立卵巢摘除术补充雌激素(OEP)大鼠模型后随机分为对照组(n=20)和实验组(n=20),分别于侧脑室微量注射生理盐水和GnIH 4μl;每组根据注射时间不同分为注射1h、2h、4h、6四个亚组(n=5)。采用蛋白印迹技术检测下丘脑POMC蛋白表达的情况。结果:注射1h、2h、4h,实验组大鼠下丘脑POMC蛋白的表达量与对照组比较,差异无统计学意义(P>0.05)。注射6h,实验组大鼠下丘脑POMC蛋白的表达量明显低于对照组(P<0.05)。结论:侧脑室微量注射GnIH可以抑制下丘脑POMC蛋白的表达。Objective： To explore the effects of microinjection of gonadotropin releasing hormone（GnIH） into lateral ventricle on proopiomelanocortin（POMC） expression in hypothalamus of ovariectomized and estrogen-primed（OEP） rats.Methods： 40 female SD rats were established OEP rat＇s model and then randomly divided into control group and experimental group with 20 rats in each group. The rats in 2 groups were injected 4μl normal saline and 4μl GnIH into the lateral ventricle respectively; and the rats in each group were further divided into 1 h, 2 h, 4 h, 6 h 4 subgroups according to the time after injection（n=5）. Western blot was used to detect the POMC protein expression in hypothalamus of rats in each group. Results： 1 h, 2 h, 4 h after injection, there was no statistically significant difference about POMC expression in hypothalamus of rats in experimental group compared with that of rats in control group（P〈0.05）. 6 h after injection, the POMC expression in hypothalamus of rats in experimental group was obviously lower than that of rats in control group（P〈0.05）. Conclusions： Microinjection of GnIH into the lateral ventricle can inhibit expression of POMC in hypothalamus.

关 键 词：侧脑室 GNIH POMC 下丘脑-垂体-性腺轴 

分 类 号：R338.2[医药卫生—人体生理学]