D-苯基乳酸高产菌株发酵罐发酵条件研究  

Study on Fermentation Conditions of High Yield Strain of D-Phenyllactic Acid in Fermentation Tank

作　　者：谢建松[1] 徐艳[2] 郑丽雪[1] 

机构地区：[1]常熟理工学院生物与食品工程学院,江苏常熟215500 [2]苏州大学基础医学与生物科学学院,江苏苏州215000

出　　处：《食品研究与开发》2017年第21期173-177,共5页Food Research and Development

基　　金：常熟理工学院重点毕业论文团队项目

摘　　要：研究了D-苯基乳酸高产菌株在5 L发酵罐中全细胞转化苯丙酮酸钠合成D-苯基乳酸的条件,分别考察了溶氧量、表面活性剂、底物补加方式等对D-苯基乳酸产量的影响。结果表明:最适溶氧为40%,控制p H值为7.0分批补加底物得到D-苯基乳酸的产量为18.76 g/L,较未控制p H值发酵提高7.22%;添加1%吐温80时,D-苯基乳酸产量达到了18.59 g/L,比未加表面活性剂发酵提高了6.98%。在此基础上,将底物匀速流加得到D-苯基乳酸的产量提高到19.43 g/L。The conditions of the synthesis of D-phenyllactic acid by the whole cell transformation of phenylpyruvic acid in 5 L fermentor were studied in this paper. Dissolved oxygen, surfactant, substrate adding method on the yield of D-phenyllactic acid were studied. The results showed： the optimum dissolved oxygen was 40 %, the yield of D-phenyllactic acid was 18.76 g/L when batch addition of the substrate and a constant pH of 7.0, compared with natural pH fermentation increased by 7.22 %. The yield of D-phenyllactic acid was up to 18.59 g/L when 1 % Twain was added, compared with that without surfactant addition increased by 6.98 %. On the basis of this, the yield of D-phenyllactic acid was increased to 19.43 g/L under the uniform substrate flow.

关 键 词：大肠杆菌 D-苯基乳酸 发酵罐 全细胞转化 

分 类 号：TS201.3[轻工技术与工程—食品科学]