MK801抑制人结肠癌裸鼠移植瘤的实验研究  

Experimental Study of MK801 in Inhibiting Human Colon Cancer Xenograft in Nude Mice

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作  者:李雪[1] 郭玉婷[2] 

机构地区:[1]山东中医药大学,济南250355 [2]山东中医药大学第二附属医院,济南250001

出  处:《中西医结合研究》2017年第3期132-135,共4页Research of Integrated Traditional Chinese and Western Medicine

摘  要:目的研究NMDA受体1(NMDA receptor1,NMDAR1)抑制剂MK801对人结肠癌裸鼠移植瘤生长的抑制作用及其机制。方法建立人结肠癌HT-29细胞株裸鼠移植瘤模型,分为实验组和对照组,实验组给予MK801 0.6mg/(kg·d)腹腔注射给药,对照组腹腔注射等量生理盐水,给药4周后处死裸鼠并称取瘤重,采用免疫组织化学法检测2组肿瘤组织中NMDAR1的表达变化。结果实验组裸鼠的肿瘤体积明显小于对照组,差异有统计学意义(P<0.05)。与对照组比较,实验组肿瘤组织中NMDAR1的表达明显减少(P<0.01)。结论 NMDAR1抑制剂MK801通过抑制NMDAR1的表达来抑制人结肠癌裸鼠移植瘤的生长。Objective To study the inhibitory effect of NMDA receptor1(NMDAR1)inhibitor MK801 on the growth of human colon cancer xenografts in nude mice and its mechanism.Methods The nude mice model of human colon cancer HT-29 cell line was established and divided into experimental group and control group.The experimental group was given MK801 0.6 mg/(kg·d)intraperitoneally for 4 weeks.At the same time,the control group was given intraperitoneal injection of equal volume of normal saline.The expression of NMDAR1 was detected using immunohistochemistry in both two groups.Results After 4 weeks of intervention,the tumor volume in the experimental group was significantly smaller than that in the control group(P〈0.05).Compared with the control group,the expression of NMDAR1 in tumor tissue in experimental group decreased significantly(P〈0.01).Conclusion NMDAR1 inhibitor MK801 can inhibit the growth of human colon cancer xenograft in nude mice through inhibiting the expression of NMDAR1.

关 键 词:NMDAR1抑制剂 MK801 结肠癌 裸鼠 移植瘤 

分 类 号:R735.3[医药卫生—肿瘤]

 

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