大孔吸附树脂纯化海地瓜总皂苷的研究  

Macroporous resin purification of saponins from the sea cucumber Acaudina molpadioides

作　　者：阮伟达 刘秋凤[2] 苏永昌[2] 

机构地区：[1]福建省南安市食品药品监督管理局,福建南安362300 [2]福建省水产研究所国家海水鱼类加工技术研发分中心(厦门)福建省海洋生物增养殖与高值化利用重点实验室福建省海洋生物资源开发利用协同创新中心,福建厦门361013

出　　处：《渔业研究》2017年第5期357-364,共8页Journal of Fisheries Research

基　　金：福建省海洋高新产业发展专项项目[2013]026号;福建省省属公益类科研院所基本科研专项(2016R1003-12)

摘　　要：本文研究了海地瓜总皂苷的纯化工艺,并采用3种大孔吸附树脂对海地瓜皂苷的吸附作用进行了比较分析,筛选出最佳的吸附树脂,并对该树脂对海地瓜皂苷的静态吸附和动态吸附效果进行测定。结果表明,筛选出的D101大孔树脂为最合适的树脂型号;以D101大孔树脂静态吸附条件进行纯化,获得皂苷纯度为51.4%;以D101大孔树脂对海地瓜粗提液进行动态吸附研究,获得皂苷纯度达71.3%。因此,D101大孔树脂能够有效地应用于海地瓜总皂苷的富集和纯化。The method of purification of total saponins from Acaudina molpadioides with macroporous resin was studied. Through the comparison of 3 types of macroporous resins, the optimal absorbent material was screened and the adsorption and desorption capacity towards total saponins were investigated. The results showed that D101 macroporous resin was screened for the most suitable resin type to purify total saponins. The best condition of static adsorption-elution technology of D101 macroporous resin was obtained and the saponins purity was 51. 4%. The best condition of dynamic adsorption-elution technology of D101 macroporous resin was also investigated,and the saponins purity was 71. 3%. It was suggested that the method of D101 macroporous resin to adsorb and purify total saponins in Acaudina molpadioides was feasible and effective.

关 键 词：海地瓜 皂苷 大孔树脂 纯化 

分 类 号：R931.77[医药卫生—生药学]