白簕多糖的分离纯化及抗氧化活性研究  

Isolation, purification and anti-oxidant activity of polysaccharides from Acanthopanax trifoliatus

作　　者：程轩轩[1] 张旭红[1] 杨慧文[1] 张冰心 潘育方[1] CHENG Xuan-xuan ZHANG Xu-hong YANG Hui-wen ZHANG Bing-xin PAN Yu-fang(Key Laboratory of State Administration of Traditional Chinese Medicine for Production ＆ Development of Cantonese Medicinal Materials, Guangdong Pharmaceutical University, Guangzhou 510006, China)

机构地区：[1]广东药科大学国家中医药管理局岭南药材生产与开发重点研究室,广东广州510006

出　　处：《中草药》2017年第20期4219-4223,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81503217);广东省科技计划项目(2016ZC0172);广东省自然科学基金(2017A030313623)

摘　　要：目的提取、分离和纯化白簕多糖(ATP),测定单糖组成和相对分子质量(M)分布,并进行体外抗氧化活性检测。方法通过水提醇沉、Sevage法、DEAE-cellulose 52和Sephadex G-50柱色谱法分离、纯化ATP。采用HPLC和高校凝胶渗透色谱(HPGPC)法对多糖的单糖组成和相对分子质量分布进行测定。通过体外清除DPPH·、ABTS^+·、羟基自由基(·OH)和超氧阴离子(O_2^-·)自由基的方法,评价ATP的抗氧化活性。结果获得1个量较高的中性均一多糖(ATP1-1)以及2个酸性多糖组分ATP2、ATP3。ATP1-1主要由葡萄糖和少量半乳糖、甘露糖、鼠李糖组成。ATP2主要由葡萄糖醛酸、葡萄糖、半乳糖、阿拉伯糖和少量甘露糖、鼠李糖组成。ATP3主要由鼠李糖、葡萄糖醛酸、葡萄糖、半乳糖和阿拉伯糖组成。ATP1-1的重均相对分子质量(Mw)为2 310。抗氧化实验表明ATP1-1对DPPH·、ABTS^+·、·OH和O_2^-·自由基均有明显的清除作用,半数抑制浓度(IC_(50))分别为0.042 4、0.007 9、2.313 6、1.753 0 mg/m L。结论 ATP1-1有较好的抗氧化活性并呈现出良好的量效关系。Objective To extract, isolate and purify the polysaccharides from Acanthopanax trifoliatus（ATP）, determine the monosaccharide composition and molecular weight distribution of ATP, and evaluate its anti-oxidant activity in vitro. Methods ATPs were prepared by water extraction ethanol precipitation. Sevage deproteinization, DEAE-cellulose 52, and Sephadex G-50 column chromatography were applied for the further isolation and purification of polysaccharides. Then, the monosaccharide composition and mean molecular mass of ATP were analyzed by HPLC and HPGPC. The antioxidant activity of ATP was evaluated through the scavenging capabilities of DPPH·, ABTS^＋·, ·OH and O2^-·free radicals. Results A neutral homogeneous polysaccharide（ATP1-1） and two acidic polysaccharides（ATP2 and ATP3） were obtained by column chromatography. According to the HPLC analysis, ATP1-1 consists of glucose, galactose, mannose and rhamnose. ATP2 consists of glucuronic acid, glucose, galactose, arabinose, mannose, and rhamnose. ATP3 was composed of rhamnose, glucuronic acid, glucose, galactose, and arabinose. ATP1-1 with the average molecular mass of 2 310, had obvious effect on scavenging ability of DPPH·, ABTS^＋·, ·OH and O2^-·free radicals, with IC50 values of 0.042 4, 0.007 9, 2.313 6, and 1.753 0 mg/mL, respectively. Conclusion ATP1-1 displayed obvious anti-oxidant activity with a good dose-effect relationship.

关 键 词：白簕 多糖 抗氧化活性 中性均一多糖 单糖组成 

分 类 号：R284.2[医药卫生—中药学]