RP-HPLC检测不同pH条件下毛诃子中单宁酸等4种多酚化合物含量变化  

Determination of Content Changes of Four Tannin Polyphenolic Compounds in Fructus Terminaliae Billericae by RP-HPLC under Different pH Conditions

作　　者：马定乾 陈平[1] 华杰[1] 左晓霜 范源[1] 

机构地区：[1]云南中医学院,云南昆明650500

出　　处：《云南中医中药杂志》2017年第10期72-75,共4页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

摘　　要：目的应用RP-HPLC法分析在不同的pH溶液中,毛诃子中Tannins、GA、β-PGG及Corilagin的含量变化。方法将毛诃子粉末分别采取回流提取方法的产物加入pH为2-7缓冲液,用反相高效液相色谱测定单宁酸、没食子酸、β-PGG及柯里拉京的含量并分析。结果毛诃子中单宁酸含量随pH增大而增加;没食子酸含量随pH增大而变化不明显;β-PGG含量随pH增大而减小;柯里拉京含量随pH增大而减小。结论通过研究不同pH条件下毛诃子中的Tannins、GA、β-PGG及Corilagin的含量变化,可为毛诃子中相关成分的质控提供一定理论参考。Objective： To analyze the contents of tannins, agllic acid, β-PGG and corilagin in Fructus Terminaliae Billericae in dif- ferent pH solutions by using RP - HPLC. Methods ： The products extracted from Terminaliae Billericae powder by reflux extraction were joined with 2 to 7 pH buffer solution and the contents of tannic acid, gallic acid, β - PGG and corilagin were determined and analyzed by RP- HPLC. Results：The content of tannic acid increased with the increase of pH. The content of gallic acid changed unapparently with the increase of pH. The content of β - PGG decreased with the increase of pH. The content of corilagin decreased with the increase of pH. Conclusion： By studying the content changes of tannins, GA, β- PGG and corilagin in Fructus Terminaliae Billericae under different pH conditions, we can provide some theoretical reference for the quality control of related components in Fructus Terminaliae Billericae.

关 键 词：毛诃子 单宁酸 没食子酸 β—PGG 柯里拉京 RP—HPLC 

分 类 号：R927.2[医药卫生—药学]